Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Geobacillus kaustophilus |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.1 M imidazole hydrochloric acid pH 8.0, 1 M sodium citrate tribasic | Geobacillus kaustophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required for activity | Geobacillus kaustophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56200 | - |
6 * 56200, calculated from amino acid sequence | Geobacillus kaustophilus |
58000 | - |
6 * 58000, His-tagged enzyme, SDS-PAGE | Geobacillus kaustophilus |
321000 | - |
gel filtration | Geobacillus kaustophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arabinose | Geobacillus kaustophilus | - |
L-ribulose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus kaustophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography and Superdex 200 gel filtration | Geobacillus kaustophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.84 | - |
in the absence of Mn2+, at pH 7.5 and 60°C | Geobacillus kaustophilus |
4.03 | - |
in the presence of Mn2+, at pH 7.5 and 60°C | Geobacillus kaustophilus |
4.57 | - |
purified enzyme, at pH 7.5 and 60°C | Geobacillus kaustophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arabinose | - |
Geobacillus kaustophilus | L-ribulose | - |
? |
Subunits | Comment | Organism |
---|---|---|
homohexamer | 6 * 56200, calculated from amino acid sequence | Geobacillus kaustophilus |
homohexamer | 6 * 58000, His-tagged enzyme, SDS-PAGE | Geobacillus kaustophilus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Geobacillus kaustophilus | calculated from amino acid sequence | - |
5.4 |