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Literature summary for 5.3.1.4 extracted from
- A novel L-arabinose isomerase from Lactobacillus fermentum CGMCC2921 for D-tagatose production: Gene cloning, purification and characterization (2011), J. Mol. Catal. B, 70, 1-7.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Limosilactobacillus fermentum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 20% residual activity at 1 mM | Limosilactobacillus fermentum |  |
| EDTA | complete inhibition at 1 mM | Limosilactobacillus fermentum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 29.9 | - |
L-arabinose | in 50 mM phosphate buffer (pH 6.5), 1mM Mn2+, 2mM Co2+, at 65°C | Limosilactobacillus fermentum | |
| 60.2 | - |
D-galactose | in 50 mM phosphate buffer (pH 6.5), 1mM Mn2+, 2mM Co2+, at 65°C | Limosilactobacillus fermentum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 120% activity at 1 mM | Limosilactobacillus fermentum | |
| Co2+ | 201% activity at 1 mM, Co2+ is required for enzymatic activity and thermostability | Limosilactobacillus fermentum | |
| Mn2+ | 298% activity at 1 mM, Mn2+ is required for enzymatic activity and thermostability | Limosilactobacillus fermentum | |
| additional information | not influenced by Ca2+ and Ni2+ | Limosilactobacillus fermentum | |
| Zn2+ | 129% activity at 1 mM | Limosilactobacillus fermentum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
x * 53000, SDS-PAGE | Limosilactobacillus fermentum |
| 53428 | - |
x * 53428, calculated from amino acid sequence | Limosilactobacillus fermentum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Limosilactobacillus fermentum | D9ILD9 | - |
- |
| Limosilactobacillus fermentum CGMCC2921 | D9ILD9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA resin column chromatography | Limosilactobacillus fermentum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-galactose | 100% activity, conversion rate of 55% with 1mM Mn2+ after 12 h at 65°C | Limosilactobacillus fermentum | D-tagatose | - |
? | |
| D-galactose | 100% activity, conversion rate of 55% with 1mM Mn2+ after 12 h at 65°C | Limosilactobacillus fermentum CGMCC2921 | D-tagatose | - |
? | |
| L-arabinose | high preference for L-arabinose (220% compared to D-galactose) | Limosilactobacillus fermentum | L-ribulose | - |
r | |
| L-arabinose | high preference for L-arabinose (220% compared to D-galactose) | Limosilactobacillus fermentum CGMCC2921 | L-ribulose | - |
r | |
| additional information | D-xylose (2.7%), D-mannose (2.5%), L-xylose (1.9%), D-glucose (1.7%), and L-ribose (0.7%) do not serve as substrates in the presence of Mn2+ or Co2+ | Limosilactobacillus fermentum | ? | - |
? | |
| additional information | D-xylose (2.7%), D-mannose (2.5%), L-xylose (1.9%), D-glucose (1.7%), and L-ribose (0.7%) do not serve as substrates in the presence of Mn2+ or Co2+ | Limosilactobacillus fermentum CGMCC2921 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 53000, SDS-PAGE | Limosilactobacillus fermentum |
| ? | x * 53428, calculated from amino acid sequence | Limosilactobacillus fermentum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| L-AI | - |
Limosilactobacillus fermentum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Limosilactobacillus fermentum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
94% activity is remained at 60°C | Limosilactobacillus fermentum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | 85 | the enzyme shows half-lives of 30 min at 85°C and 220 min at 75°C | Limosilactobacillus fermentum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Limosilactobacillus fermentum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
the enzyme exhibits 83% of its maximal activity at pH 6.0 | Limosilactobacillus fermentum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6 | the enzyme retains 88% and 80% of its original activity after 24 h of incubation at pH 6.0 and 5.5, respectively. At pH 5.0, 55% of its activity is remained | Limosilactobacillus fermentum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Limosilactobacillus fermentum | calculated from amino acid sequence | - |
4.8 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.15 | - |
D-galactose | in 50 mM phosphate buffer (pH 6.5), 1mM Mn2+, 2mM Co2+, at 65°C | Limosilactobacillus fermentum | |
| 0.32 | - |
L-arabinose | in 50 mM phosphate buffer (pH 6.5), 1mM Mn2+, 2mM Co2+, at 65°C | Limosilactobacillus fermentum | |
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