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Literature summary for 5.3.1.4 extracted from

  • Sakakibara, Y.; Saha, B.C.; Taylor, P.
    Microbial production of xylitol from L-arabinose by metabolically engineered Escherichia coli (2009), J. Biosci. Bioeng., 107, 506-511.
    View publication on PubMed
Search for more literature for 5.3.1.4

Cloned(Commentary)

Cloned (Comment) Organism
enzyme coexpression in Escherichia coli strain ZUC99 with D-psicose 3-epimerase and L-xylulose reductase, leading to production of L-xylitol, under control of the araBAD promoter Escherichia coli K-12

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ required Escherichia coli K-12

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arabinose Escherichia coli K-12
-
 L-ribulose
-
 ?
L-arabinose Escherichia coli K-12 AB707
-
 L-ribulose
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli K-12
-
 gene araA
-
Escherichia coli K-12 AB707
-
 gene araA
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arabinose
-
 Escherichia coli K-12 L-ribulose
-
 ?
L-arabinose
-
 Escherichia coli K-12 AB707 L-ribulose
-
 ?

Synonyms

Synonyms Comment Organism
AraA
-
 Escherichia coli K-12

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Escherichia coli K-12

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Escherichia coli K-12

Expression

Organism Comment Expression
Escherichia coli K-12 production of xylitol from L-arabinose by metabolically engineered Escherichia coli enzyme expressing three enzymes in strain ZUC99: L-arabinose isomerase, which converts L-arabinose to L-ribulose, D-psicose 3-epimerase, which converts L-ribulose to L-xylulose, and L-xylulose reductase, which converts L-xylulose to xylitol, method optimization, overview additional information