Cloned (Comment) | Organism |
---|---|
enzyme coexpression in Escherichia coli strain ZUC99 with D-psicose 3-epimerase and L-xylulose reductase, leading to production of L-xylitol, under control of the araBAD promoter | Escherichia coli K-12 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required | Escherichia coli K-12 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arabinose | Escherichia coli K-12 | - |
L-ribulose | - |
? | |
L-arabinose | Escherichia coli K-12 AB707 | - |
L-ribulose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli K-12 | - |
gene araA | - |
Escherichia coli K-12 AB707 | - |
gene araA | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arabinose | - |
Escherichia coli K-12 | L-ribulose | - |
? | |
L-arabinose | - |
Escherichia coli K-12 AB707 | L-ribulose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AraA | - |
Escherichia coli K-12 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Escherichia coli K-12 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli K-12 |
Organism | Comment | Expression |
---|---|---|
Escherichia coli K-12 | production of xylitol from L-arabinose by metabolically engineered Escherichia coli enzyme expressing three enzymes in strain ZUC99: L-arabinose isomerase, which converts L-arabinose to L-ribulose, D-psicose 3-epimerase, which converts L-ribulose to L-xylulose, and L-xylulose reductase, which converts L-xylulose to xylitol, method optimization, overview | additional information |