Cloned (Comment) | Organism |
---|---|
gene araA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) | Acidothermus cellulolyticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 30% inhibition at 1 mM | Acidothermus cellulolyticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates and increase thermostability of the enzyme | Acidothermus cellulolyticus | |
Mn2+ | activates and increase thermostability of the enzyme | Acidothermus cellulolyticus | |
additional information | metal ions are essential for catalytic activity, but metal ions, such as Mg2+, Ni2+, and Ba2+, are poor activators, while Fe2+, Ca2+, and Zn2+ have no effect on the activity | Acidothermus cellulolyticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54768 | - |
x * 54768, sequence calculation, x * 55000, recombinant enzyme, SDS-PAGE | Acidothermus cellulolyticus |
55000 | - |
x * 54768, sequence calculation, x * 55000, recombinant enzyme, SDS-PAGE | Acidothermus cellulolyticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arabinose | Acidothermus cellulolyticus | - |
L-ribulose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidothermus cellulolyticus | A0LT86 | ATCC 43068, gene araA | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3) by heat treatment, ion exchange chromatography, and gel filtration | Acidothermus cellulolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose | conversion yield over 50% after 12 h under optimal conditions | Acidothermus cellulolyticus | D-tagatose | - |
? | |
L-arabinose | - |
Acidothermus cellulolyticus | L-ribulose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 54768, sequence calculation, x * 55000, recombinant enzyme, SDS-PAGE | Acidothermus cellulolyticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Acidothermus cellulolyticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
purified recombinant enzyme, completely stable for 2 h | Acidothermus cellulolyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Acidothermus cellulolyticus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | over 90% of maximal activity at pH 6.0, maximal activity at pH 7.5 | Acidothermus cellulolyticus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
25°C, purified recombinant enzyme, 80% activity remaining after 12 h, 70% after 24 h | Acidothermus cellulolyticus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Acidothermus cellulolyticus | sequence calculation | - |
5.63 |