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Literature summary for 5.3.1.4 extracted from
- Cloning and characterization of a novel L-arabinose isomerase from Bacillus licheniformis (2008), Appl. Microbiol. Biotechnol., 81, 283-290.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Bacillus licheniformis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | significant inhibition | Bacillus licheniformis |  |
| Hg2+ | significant inhibition | Bacillus licheniformis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 369 | - |
L-arabinose | pH 7.5, 50°C | Bacillus licheniformis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | or Mn2+, required. 443% of initial activity at 1 mM | Bacillus licheniformis | |
| Mg2+ | 240% of initial activity at 1 mM | Bacillus licheniformis | |
| Mn2+ | or Co2+, required. 497% of initial activity at 1 mM | Bacillus licheniformis | |
| additional information | no stimulation by Ca2+, Ba2+, Fe2+, Hg2+, or Cu2+ at 1 mM each | Bacillus licheniformis | |
| Zn2+ | 307% of initial activity at 1 mM | Bacillus licheniformis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
2 * 53500, calculated, 2 * 53000, SDS-PAGE | Bacillus licheniformis |
| 53500 | - |
2 * 53500, calculated, 2 * 53000, SDS-PAGE | Bacillus licheniformis |
| 113000 | - |
gel filtration | Bacillus licheniformis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus licheniformis | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-galactose | 2% of the activity with L-arabinose | Bacillus licheniformis | D-tagatose | - |
? | |
| L-arabinose | - |
Bacillus licheniformis | L-ribulose | - |
? | |
| additional information | no substrate: L-ribose, L-xylose, D-glucose, D-mannose, D-xylose, and D-allose | Bacillus licheniformis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 53500, calculated, 2 * 53000, SDS-PAGE | Bacillus licheniformis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Bacillus licheniformis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
in presence of 1 Mm Mn2+, stable for more than 48 h | Bacillus licheniformis |
| 60 | - |
half-life 120 min | Bacillus licheniformis |
| 70 | - |
half-life 20 min | Bacillus licheniformis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 207 | - |
L-arabinose | pH 7.5, 50°C | Bacillus licheniformis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Bacillus licheniformis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | more than 80% of maximum activity | Bacillus licheniformis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bacillus licheniformis | calculated | - |
4.8 |
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