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Literature summary for 5.3.1.4 extracted from
- Distinct metal dependence for catalytic and structural functions in the L-arabinose isomerases from the mesophilic Bacillus halodurans and the thermophilic Geobacillus stearothermophilus (2005), Arch. Biochem. Biophys., 434, 333-343.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Geobacillus stearothermophilus |
| expression in Escherichia coli | Halalkalibacterium halodurans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 36 | - |
L-arabinose | - |
Halalkalibacterium halodurans |  |
| 63 | - |
L-arabinose | - |
Geobacillus stearothermophilus | |
| 117 | - |
D-galactose | - |
Geobacillus stearothermophilus | |
| 167 | - |
D-galactose | - |
Halalkalibacterium halodurans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 60% activation compared to Mn2+ | Geobacillus stearothermophilus | |
| Mg2+ | 50% activation compared to Mn2+ | Geobacillus stearothermophilus | |
| Mn2+ | approximately 0.06 eqiuvalents of Mn2+/subunit | Halalkalibacterium halodurans | |
| Mn2+ | strong activator, approximately 0.06 eqiuvalents of Mn2+/subunit | Geobacillus stearothermophilus | |
| additional information | the conversion of D-galactose into D-tagatose is not dependent on divalent metal ions | Halalkalibacterium halodurans |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56090 | - |
monomer, calculated from amino acid sequence | Geobacillus stearothermophilus |
| 56320 | - |
monomer, calculated from amino acid sequence | Halalkalibacterium halodurans |
| 57000 | - |
monomer, SDS-PAGE | Geobacillus stearothermophilus |
| 57000 | - |
monomer, SDS-PAGE | Halalkalibacterium halodurans |
| 112000 | - |
native gradient gel electrophoresis, weak band (dimer) | Geobacillus stearothermophilus |
| 112000 | - |
native gradient gel electrophoresis, weak band (dimer) | Halalkalibacterium halodurans |
| 224000 | - |
native gradient gel electrophoresis | Geobacillus stearothermophilus |
| 224000 | - |
native gradient gel electrophoresis | Halalkalibacterium halodurans |
| 225000 | - |
gel filtration | Geobacillus stearothermophilus |
| 225000 | - |
gel filtration | Halalkalibacterium halodurans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-galactose | Geobacillus stearothermophilus | at elevated temperatures in the presence of divalent metal ions | D-tagatose | - |
? | |
| D-galactose | Halalkalibacterium halodurans | at elevated temperatures, not dependent on divalent metal ions | D-tagatose | - |
? | |
| L-arabinose | Geobacillus stearothermophilus | - |
L-ribulose | - |
? | |
| L-arabinose | Halalkalibacterium halodurans | - |
L-ribulose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
- |
- |
| Halalkalibacterium halodurans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme, to homogeneity | Geobacillus stearothermophilus |
| recombinant enzyme, to homogeneity | Halalkalibacterium halodurans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.3 | - |
with D-galactose as substrate | Halalkalibacterium halodurans |
| 9 | - |
with D-galactose as substrate | Geobacillus stearothermophilus |
| 33.1 | - |
with L-arabinose as substrate | Halalkalibacterium halodurans |
| 36.5 | - |
with L-arabinose as substrate | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-galactose | at elevated temperatures in the presence of divalent metal ions | Geobacillus stearothermophilus | D-tagatose | - |
? | |
| D-galactose | at elevated temperatures, not dependent on divalent metal ions | Halalkalibacterium halodurans | D-tagatose | - |
? | |
| L-arabinose | - |
Geobacillus stearothermophilus | L-ribulose | - |
? | |
| L-arabinose | - |
Halalkalibacterium halodurans | L-ribulose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 57000, SDS-PAGE, gel filtration | Geobacillus stearothermophilus |
| tetramer | 4 * 57000, SDS-PAGE, gel filtration | Halalkalibacterium halodurans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AI | - |
Geobacillus stearothermophilus |
| AI | - |
Halalkalibacterium halodurans |
| L-arabinose isomerase | - |
Geobacillus stearothermophilus |
| L-arabinose isomerase | - |
Halalkalibacterium halodurans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Halalkalibacterium halodurans |
| 70 | - |
- |
Geobacillus stearothermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
EDTA-treated AI has a lower melting temperature (72°C) than the holoenzyme (78°C) | Geobacillus stearothermophilus |
| additional information | - |
Tm is 65-67°C, unaffected by the presence of metal ions | Halalkalibacterium halodurans |
| 70 | - |
half life: 20 min | Halalkalibacterium halodurans |
| 80 | - |
half life: 52 min | Geobacillus stearothermophilus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.2 | - |
D-galactose | - |
Halalkalibacterium halodurans | |
| 8.4 | - |
D-galactose | - |
Geobacillus stearothermophilus | |
| 31 | - |
L-arabinose | - |
Halalkalibacterium halodurans | |
| 34.1 | - |
L-arabinose | - |
Geobacillus stearothermophilus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | - |
Geobacillus stearothermophilus |
| 8 | - |
at 50°C and in the presence of 1 mM Mn2+ | Halalkalibacterium halodurans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | - |
Geobacillus stearothermophilus |
| 6 | 10 | - |
Halalkalibacterium halodurans |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
- |
5.6 |
| Halalkalibacterium halodurans | - |
- |
5.8 |
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