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Literature summary for 5.3.1.4 extracted from
- L-Arabinose isomerase from Lactobacillus gayonii (1975), Methods Enzymol., 41B, 458-461.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Lactobacillus gayonii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Lactobacillus gayonii |  |
| Fe2+ | - |
Lactobacillus gayonii | |
| Hg2+ | - |
Lactobacillus gayonii | |
| L-arabitol | - |
Lactobacillus gayonii | |
| ribitol | - |
Lactobacillus gayonii | |
| Zn2+ | - |
Lactobacillus gayonii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 55 | - |
L-arabinose | - |
Lactobacillus gayonii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activates to about half the extent of Mn2+ | Lactobacillus gayonii | |
| K+ | slight activation | Lactobacillus gayonii | |
| Mn2+ | required | Lactobacillus gayonii | |
| Sr2+ | slight activation | Lactobacillus gayonii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 271000 | - |
sucrose density gradient centrifugation | Lactobacillus gayonii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactobacillus gayonii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lactobacillus gayonii |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| beta-L-arabinopyranose = L-ribulose | compulsory ordered mechanism that involves the formation of a ternary complex of enzyme-Mn-substrate | Lactobacillus gayonii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.31 | - |
- |
Lactobacillus gayonii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-arabinose | - |
Lactobacillus gayonii | L-ribulose | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
pH 5.5-9.0, 10 min, stable | Lactobacillus gayonii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | - |
Lactobacillus gayonii |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | 50°C, 10 min, stable | Lactobacillus gayonii |
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