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Literature summary for 5.3.1.4 extracted from
- L-Arabinose isomerase (1975), Methods Enzymol., 41B, 453-458.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| L-arabitol | - |
Escherichia coli |  |
| ribitol | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 60 | - |
L-arabinose | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | does not restore activity after dialysis with EDTA | Escherichia coli | |
| Mn2+ | restores activity after dialysis against EDTA | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
6 * 60000, sedimentation in the ultracentrifuge in presence of 8 M urea or pH 2 phosphate buffer | Escherichia coli |
| 362000 | - |
low speed equilibrium sedimentation | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli | - |
temperature-sensitive mutant | - |
| Escherichia coli B/r | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, or lyophilized and stored at -20°C, stable for at least 1 month | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Fucose | slightly less than 10% of the activity on L-arabinose | Escherichia coli | ? | - |
? | |
| D-Fucose | slightly less than 10% of the activity on L-arabinose | Escherichia coli B/r | ? | - |
? | |
| D-Xylose | slightly less than 10% of the activity on L-arabinose | Escherichia coli | ? | - |
? | |
| D-Xylose | slightly less than 10% of the activity on L-arabinose | Escherichia coli B/r | ? | - |
? | |
| L-arabinose | - |
Escherichia coli | L-ribulose | - |
? | |
| L-arabinose | - |
Escherichia coli B/r | L-ribulose | - |
? | |
| L-Fucose | slightly less than 10% of the activity on L-arabinose | Escherichia coli | ? | - |
? | |
| L-Fucose | slightly less than 10% of the activity on L-arabinose | Escherichia coli B/r | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 60000, sedimentation in the ultracentrifuge in presence of 8 M urea or pH 2 phosphate buffer | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | - |
Escherichia coli |
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Release 2023.1 (January 2023)
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