Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.3.1.29 extracted from

  • Aono, R.; Sato, T.; Yano, A.; Yoshida, S.; Nishitani, Y.; Miki, K.; Imanaka, T.; Atomi, H.
    Enzymatic characterization of AMP phosphorylase and ribose-1,5-bisphosphate isomerase functioning in an archaeal AMP metabolic pathway (2012), J. Bacteriol., 194, 6847-6855.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.3.1.29

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
 alpha-D-ribose 1,5-bisphosphate pH 8.3, 85°C Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-D-ribose 1,5-bisphosphate Thermococcus kodakarensis the archaeal enzyme is involved in AMP metabolism and CO2 fixation through type III RubisCO enzymes D-ribulose 1,5-bisphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Thermococcus kodakarensis Q5JFM9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-ribose 1,5-bisphosphate the archaeal enzyme is involved in AMP metabolism and CO2 fixation through type III RubisCO enzymes Thermococcus kodakarensis D-ribulose 1,5-bisphosphate
-
 ?
alpha-D-ribose 1,5-bisphosphate the enzyme is specific for the alpha-anomer of D-ribose 1,5-bisphosphate and does not recognize other sugar compounds (ribose 5-phosphate, phosphoribosylpyrophosphate, fructose 1,6-bisphosphate, fructose 6-phosphate, glucose, pyruvate or 3-phosphoglycerate) Thermococcus kodakarensis D-ribulose 1,5-bisphosphate
-
 ?

Synonyms

Synonyms Comment Organism
R15P isomerase
-
 Thermococcus kodakarensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
 assay at Thermococcus kodakarensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
29.2
-
 alpha-D-ribose 1,5-bisphosphate pH 8.3, 85°C Thermococcus kodakarensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
 assay at Thermococcus kodakarensis

Cofactor

Cofactor Comment Organism Structure
AMP activity is extremely low with the substrate alpha-D-ribose 1,5-bisphosphate alone but is more than 40fold activated in the presence of AMP Thermococcus kodakarensis

Expression

Organism Comment Expression
Thermococcus kodakarensis increased protein levels of the ribose 1,5-bisphosphate isomerase in the cells cultivated with nucleosides up

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
48.7
-
 alpha-D-ribose 1,5-bisphosphate pH 8.3, 85°C Thermococcus kodakarensis