Protein Variants | Comment | Organism |
---|---|---|
additional information | fusion gene construct of 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase. The gene product of 3-hexulose-6-phosphate synthase /6-phospho-3-hexuloisomerase exhibits both activities at room temperature and catalyzes the sequential reactions more efficiently than a simple mixture of the individual enzymes. The gene product of 6-phospho-3-hexuloisomerase /3-hexulose-6-phosphate synthase fails to display any enzyme activity. Escherichia coli strains harboring the 3-hexulose-6-phosphate synthase /6-phospho-3-hexuloisomerase gene consume formaldehyde more efficiently and exhibited better growth in a formaldehyde containing medium than the host strain | Mycobacterium gastri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21000 | - |
4 * 21000, SDS-PAGE, recombinant 6-phospho-3-hexuloisomerase | Mycobacterium gastri |
42000 | - |
x * 42000, recombinant 3-hexulose-6-phosphate synthase /6-phospho-3-hexuloisomerasefusion enzyme | Mycobacterium gastri |
94000 | - |
gel filtration, recombinant 6-phospho-3-hexuloisomerase | Mycobacterium gastri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium gastri | Q9LBW5 | - |
- |
Mycobacterium gastri MB19 | Q9LBW5 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
154 | - |
3-hexulose-6-phosphate synthase/6-phospho-3-hexuloisomerasefusion enzyme, 30°C | Mycobacterium gastri |
563 | - |
6-phospho-3-hexuloisomerase, 30°C | Mycobacterium gastri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arabino-3-hexulose 6-phosphate | - |
Mycobacterium gastri | D-fructose 6-phosphate | - |
? | |
D-arabino-3-hexulose 6-phosphate | - |
Mycobacterium gastri MB19 | D-fructose 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, recombinant 3-hexulose-6-phosphate synthase /6-phospho-3-hexuloisomerasefusion enzyme | Mycobacterium gastri |
tetramer | 4 * 21000, SDS-PAGE, recombinant 6-phospho-3-hexuloisomerase | Mycobacterium gastri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
both recombinant 6-phospho-3-hexuloisomerase and 3-hexulose-6-phosphate synthase /6-phospho-3-hexuloisomerase fusion enzyme | Mycobacterium gastri |