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Literature summary for 5.3.1.26 extracted from
- Crystal structure and substrate specificity of D-galactose-6-phosphate isomerase complexed with substrates (2013), PLoS ONE, 8, e72902.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Lacticaseibacillus rhamnosus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| in complex with D-tagatose-6-phosphate, hanging drop vapor diffusion method, using 0.2 M potassium formate containing 20% (w/v) PEG 3350 | Lacticaseibacillus rhamnosus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C65A | inactive | Lacticaseibacillus rhamnosus |
| D8N | inactive | Lacticaseibacillus rhamnosus |
| H96A | the mutant exhibits 25fold lower activity compared with the wild type enzyme activity | Lacticaseibacillus rhamnosus |
| H9A | inactive | Lacticaseibacillus rhamnosus |
| N97A | the mutant has an activity level of approximately 30% that of the wild type enzyme | Lacticaseibacillus rhamnosus |
| T67A | the catalytic activity of the mutant is approximately 20fold lower than that of the wild type enzyme | Lacticaseibacillus rhamnosus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 15000 | - |
2 * 19000 + 1 * 15000, SDS-PAGE | Lacticaseibacillus rhamnosus |
| 19000 | - |
2 * 19000 + 1 * 15000, SDS-PAGE | Lacticaseibacillus rhamnosus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Galactose 6-phosphate | Lacticaseibacillus rhamnosus | - |
D-Tagatose 6-phosphate | - |
r |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lacticaseibacillus rhamnosus | A0A0D6UDD4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HisTrap column chromatography and Superdex 200 gel filtration | Lacticaseibacillus rhamnosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-allose | - |
Lacticaseibacillus rhamnosus | D-psicose | - |
? | |
| D-Galactose 6-phosphate | - |
Lacticaseibacillus rhamnosus | D-Tagatose 6-phosphate | - |
r | |
| D-ribose | - |
Lacticaseibacillus rhamnosus | D-ribulose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterotetramer | 2 * 19000 + 1 * 15000, SDS-PAGE | Lacticaseibacillus rhamnosus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-galactose-6-phosphate isomerase | - |
Lacticaseibacillus rhamnosus |
| LacAB | - |
Lacticaseibacillus rhamnosus |
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Release 2023.1 (January 2023)
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