Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Lactococcus lactis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
58 | - |
D-allose | - |
Lactococcus lactis | |
143 | - |
D-altrose | - |
Lactococcus lactis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no effect of Na+, K+, Mn2+, Zn2+, Ba2+, Cu2+, Co2+, Ca2+, Mg2+, Fe3+, Al3+ | Lactococcus lactis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
15000 | - |
LacA subunit, SDS-PAGE | Lactococcus lactis |
19000 | - |
LacB subunit, SDS-PAGE | Lactococcus lactis |
135500 | - |
native enzyme by gel filtration | Lactococcus lactis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactococcus lactis | P23495 | - |
- |
Purification (Comment) | Organism |
---|---|
of the recombinant protein by HiTrap Q HP column chromatography | Lactococcus lactis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.79 | - |
of the purified enzyme to produce D-allose | Lactococcus lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 D-lyxose | - |
Lactococcus lactis | D-xylulose + D-xylose | - |
r | |
2 D-psicose | - |
Lactococcus lactis | D-allose + D-altrose | - |
r | |
2 L-lyxose | - |
Lactococcus lactis | L-xylulose + L-xylose | - |
r | |
D-allose | - |
Lactococcus lactis | D-psicose + D-altrose | - |
r | |
D-altrose | - |
Lactococcus lactis | D-psicose + D-allose | - |
r | |
D-arabinose | - |
Lactococcus lactis | D-ribulose + D-ribose | - |
r | |
D-fructose | - |
Lactococcus lactis | D-glucose + D-mannose | - |
r | |
D-galactose | - |
Lactococcus lactis | D-tagatose + D-talose | - |
r | |
D-Galactose 6-phosphate | - |
Lactococcus lactis | D-Tagatose 6-phosphate | - |
r | |
D-glucose | - |
Lactococcus lactis | D-fructose + D-mannose | - |
r | |
D-glucose | - |
Lactococcus lactis | D-sorbose + D-idose | - |
r | |
D-mannose | - |
Lactococcus lactis | D-fructose + D-glucose | - |
r | |
D-ribose | - |
Lactococcus lactis | D-ribulose + D-arabinose | - |
r | |
D-ribulose | - |
Lactococcus lactis | D-ribose + D-arabinose | - |
r | |
D-sorbose | - |
Lactococcus lactis | D-glucose + D-idose | - |
r | |
D-talose | - |
Lactococcus lactis | D-tagatose | - |
r | |
D-Xylose | - |
Lactococcus lactis | D-Xylulose | - |
r | |
D-xylulose | - |
Lactococcus lactis | D-xylose + D-lyxose | - |
r | |
L-fructose | - |
Lactococcus lactis | L-glucose + L-mannose | - |
r | |
L-galactose | - |
Lactococcus lactis | L-tagatose + L-talose | - |
r | |
L-glucose | - |
Lactococcus lactis | L-fructose | - |
r | |
L-ribulose | - |
Lactococcus lactis | L-ribose + L-arabinose | - |
r | |
L-tagatose | - |
Lactococcus lactis | L-galactose + L-talose | - |
r | |
L-Xylose | - |
Lactococcus lactis | L-Xylulose | - |
r | |
L-xylulose | - |
Lactococcus lactis | L-xylose + L-lyxose | - |
r |
Subunits | Comment | Organism |
---|---|---|
octamer | hetero-octamer, LacA and LacB subunits | Lactococcus lactis |
Synonyms | Comment | Organism |
---|---|---|
galactose-6-phosphate isomerase | - |
Lactococcus lactis |
LacAB | - |
Lactococcus lactis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Lactococcus lactis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Lactococcus lactis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Lactococcus lactis |