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Literature summary for 5.3.1.26 extracted from
- Lactose and D-galactose metabolism in Staphylococcus aureus. II. Isomerization of D-galactose 6-phosphate to D-tagatose 6-phosphate by a specific D-galactose-6-phosphate isomerase (1980), J. Biol. Chem., 255, 8740-8744.
General Stability
| General Stability | Organism |
|---|---|
| 2-mercaptoethanol stabilizes the enzyme during purification | Staphylococcus aureus |
| completely unstable to freezing in phosphate buffer, pH 7.5, even in the presence of 15% v/v glycerol | Staphylococcus aureus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NEM | - |
Staphylococcus aureus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.9 | - |
D-tagatose 6-phosphate | - |
Staphylococcus aureus | |
| 9.6 | - |
D-galactose-6-phosphate | - |
Staphylococcus aureus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 99000 | 100000 | sucrose density gradient centrifugation, gel filtration | Staphylococcus aureus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Galactose 6-phosphate | Staphylococcus aureus | inducible enzyme in catabolism of lactose and D-galactose | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus aureus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Staphylococcus aureus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for several weeks | Staphylococcus aureus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Galactose 6-phosphate | r | Staphylococcus aureus | D-Tagatose 6-phosphate | - |
? | |
| D-Galactose 6-phosphate | inducible enzyme in catabolism of lactose and D-galactose | Staphylococcus aureus | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | 9.2 | - |
Staphylococcus aureus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
completely unstable at pH values below pH 6.5 | Staphylococcus aureus |
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Release 2023.1 (January 2023)
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