Cloned (Comment) | Organism |
---|---|
cloning of trpF mutants, expression in Escherichia coli BL21(DE3) | Thermotoga maritima |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method | Thermotoga maritima |
X-ray structure of a complex between TrpF and its product analogue reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | Thermotoga maritima |
Protein Variants | Comment | Organism |
---|---|---|
C7A | catalytically inactive mutant | Thermotoga maritima |
D126N | mutant with drastically reduced activity | Thermotoga maritima |
D126N/C7A | catalytically inactive double mutant | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00028 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | pH 7.5, 25°C, dimer | Thermotoga maritima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-(5-phospho-beta-D-ribosyl)anthranilate | Thermotoga maritima | involved in tryptophan biosynthesis from chorismate | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | Q56320 | monofunctional enzyme | - |
Purification (Comment) | Organism |
---|---|
TrpF mutants C7A, D126N and C7A/D126N double mutant | Thermotoga maritima |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | catalytic mechanism | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-(5-phospho-beta-D-ribosyl)anthranilate | high catalytic efficiency | Thermotoga maritima | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
N-(5-phospho-beta-D-ribosyl)anthranilate | reaction mechanism involving general acid-base catalysis and a Schiff base intermediate, Cys-7 and Asp-126 are important for the reaction | Thermotoga maritima | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? | |
N-(5-phospho-beta-D-ribosyl)anthranilate | involved in tryptophan biosynthesis from chorismate | Thermotoga maritima | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
TrpF | - |
Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
extremely thermostable | Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.7 | - |
N-(5-phospho-beta-D-ribosyl)anthranilate | pH 7.5, 25°C, dimer | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Thermotoga maritima |