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Literature summary for 5.3.1.24 extracted from

  • Sterner, R.; Kleemann, G.R.; Szadkowski, H.; Lustig, A.; Hennig, M.; Kirschner, K.
    Phosphoribosyl anthranilate isomerase from Thermotoga maritima is an extremely stable and active homodimer (1996), Protein Sci., 5, 2000-2008.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.3.1.24

Cloned(Commentary)

Cloned (Comment) Organism
trpF gene is cloned and expressed in Escherichia coli Thermotoga maritima

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method Thermotoga maritima

General Stability

General Stability Organism
extremely stable enzyme Thermotoga maritima
extremely stable towards proteolytic attack Thermotoga maritima

Inhibitors

Inhibitors Comment Organism Structure
reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate binds reversibly; competitive inhibitor Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00028
-
 N-(5-phospho-beta-D-ribosyl)anthranilate pH 7.5, 25°C, dimer Thermotoga maritima
0.00039
-
 N-(5-phospho-beta-D-ribosyl)anthranilate pH 7.5, 45°C, dimer Thermotoga maritima
0.00073
-
 N-(5-phospho-beta-D-ribosyl)anthranilate pH 7.5, 60°C, dimer Thermotoga maritima
0.00103
-
 N-(5-phospho-beta-D-ribosyl)anthranilate pH 7.5, 80°C, dimer Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23040
-
 2 * 23040, calculated from the amino acid sequence Thermotoga maritima
49600
-
 dimer, sedimentation equilibrium analysis Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-(5-phospho-beta-D-ribosyl)anthranilate Thermotoga maritima fourth step in tryptophan biosynthesis 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?
N-(5-phospho-beta-D-ribosyl)anthranilate Thermotoga maritima involved in tryptophan biosynthesis from chorismate 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
 monofunctional enzyme
-

Purification (Commentary)

Purification (Comment) Organism
recombinant PRAI, expressed in Escherichia coli Thermotoga maritima

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-(5-phospho-beta-D-ribosyl)anthranilate high catalytic efficiency Thermotoga maritima 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ir
N-(5-phospho-beta-D-ribosyl)anthranilate fourth step in tryptophan biosynthesis Thermotoga maritima 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?
N-(5-phospho-beta-D-ribosyl)anthranilate involved in tryptophan biosynthesis from chorismate Thermotoga maritima 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 23040, calculated from the amino acid sequence Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
 dimer, half-life: 310 min Thermotoga maritima
91.5
-
 dimer, half-life: 50 min Thermotoga maritima
95
-
 dimer, strongly resistant toward inactivation up to 95°C, half-life: 21 min Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.7
-
 N-(5-phospho-beta-D-ribosyl)anthranilate pH 7.5, 25°C, dimer Thermotoga maritima
13.5
-
 N-(5-phospho-beta-D-ribosyl)anthranilate pH 7.5, 45°C, dimer Thermotoga maritima
38.5
-
 N-(5-phospho-beta-D-ribosyl)anthranilate pH 7.5, 60°C, dimer Thermotoga maritima
130
-
 N-(5-phospho-beta-D-ribosyl)anthranilate pH 7.5, 80°C, dimer Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Thermotoga maritima

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.2
-
 strongly resistant toward inactivation by acidification to pH 3.2 Thermotoga maritima