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Literature summary for 5.3.1.21 extracted from
- Purification and characterization of corticosteroid side chain isomerase (1990), Biochemistry, 29, 1147-1154.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Rattus norvegicus | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | stimulates | Rattus norvegicus |  |
| Ni2+ | stimulates | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23500 | - |
2 * 23500, SDS-PAGE | Rattus norvegicus |
| 44000 | - |
gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| no glycoprotein | - |
Rattus norvegicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0-4°C, 0.01 M sodium phosphate, pH 7.0, less than 10% loss of activity after 6 months | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 11-Deoxycorticosterone | - |
Rattus norvegicus | 20-Hydroxy-3-oxopregn-4-en-21-al | - |
? | |
| 17alpha-Hydroxy-11-deoxycorticosterone | - |
Rattus norvegicus | 17alpha,20-Dihydroxy-3-oxopregn-4-en-21-al | - |
? | |
| Corticosterone | - |
Rattus norvegicus | 11beta,20-Dihydroxy-3-oxopregn-4-en-21-al | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 23500, SDS-PAGE | Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
30 min, stable | Rattus norvegicus |
| 58 | - |
10 min, about 20% loss of activity | Rattus norvegicus |
| 65 | - |
10 min, about 45% loss of activity | Rattus norvegicus |
| 100 | - |
60 s, complete loss of activity | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9.6 | acetate/phosphate buffer | Rattus norvegicus |
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Release 2023.1 (January 2023)
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