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Literature summary for 5.3.1.13 extracted from

  • Airoldi, C.; Sommaruga, S.; Merlo, S.; Sperandeo, P.; Cipolla, L.; Polissi, A.; Nicotra, F.
    Targeting bacterial membranes: NMR spectroscopy characterization of substrate recognition and binding requirements of D-arabinose-5-phosphate isomerase (2010), Chemistry, 16, 1897-1902.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Zn2+ Zn2+ inhibits substrate binding in a reversible manner Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-Arabinose 5-phosphate Escherichia coli
-
D-Ribulose 5-phosphate
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli P45395
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography, gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-deoxy-D-threo-pentofuranose 5-phosphate the catalysis proceeds 10fold slower than for the natural substrate isomerization Escherichia coli 3-deoxy-D-glycero-pentulose 5-phosphate
-
?
4-deoxy-D-threo-arabinose 5-phosphate the isomerization rate for this compound is 2fold higher than that of the natural substrate Escherichia coli 4-deoxy-D-glycero-pentulose 5-phosphate
-
?
D-Arabinose 5-phosphate
-
Escherichia coli D-Ribulose 5-phosphate
-
r
additional information the enzyme does not isomerize D-xylulose-5-phosphate, D-arabinose, D-ribose, and D-ribulose Escherichia coli ?
-
?

Synonyms

Synonyms Comment Organism
API
-
Escherichia coli
D-arabinose-5-phosphate isomerase
-
Escherichia coli
KdsD
-
Escherichia coli

General Information

General Information Comment Organism
metabolism API is a key enzyme in the biosynthesis of lipopolysaccharide, it catalyzes the first reaction of 3-deoxy-d-manno-octulosonate biosynthesis Escherichia coli