Any feedback?
Literature summary for 5.3.1.13 extracted from
- Characterization of Escherichia coli D-arabinose 5-phosphate isomerase encoded by kpsF: implications for group 2 capsule biosynthesis (2006), Biochem. J., 395, 427-432.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Escherichia coli |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | - |
Escherichia coli | |
| Cu2+ | - |
Escherichia coli | |
| Hg2+ | - |
Escherichia coli | |
| Zn2+ | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.3 | - |
D-ribulose 5-phosphate | - |
Escherichia coli | |
| 0.35 | - |
D-ribulose 5-phosphate | - |
Escherichia coli | |
| 0.57 | - |
D-arabinose 5-phosphate | - |
Escherichia coli | |
| 0.61 | - |
D-arabinose 5-phosphate | - |
Escherichia coli | |
| 0.64 | - |
D-ribulose 5-phosphate | - |
Escherichia coli | |
| 1.2 | - |
D-arabinose 5-phosphate | - |
Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | weak activator | Escherichia coli | |
| Fe2+ | weak activator | Escherichia coli | |
| Mg2+ | weak activator | Escherichia coli | |
| Mn2+ | weak activator | Escherichia coli | |
| additional information | Ba2+, Co2+, Ni2+ have no effect | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 122000 | - |
gel filtration | Escherichia coli |
| 133000 | - |
gel filtration | Escherichia coli |
| 145000 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P45395 | CFT073 | - |
| Escherichia coli CFT073 | P45395 | CFT073 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| of the recombinant protein by Q-Sepharose fast flow column chromatography | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80° | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Arabinose 5-phosphate | - |
Escherichia coli | D-Ribulose 5-phosphate | no activity with D-ribose-5-phosphate, D-arabinose, D-glucose-6-phosphate, D-mannose-6-phosphate, alpha-D-glucose-1-phosphate and D-glucosamine-6-phosphate | r | |
| D-Arabinose 5-phosphate | - |
Escherichia coli | D-Ribulose 5-phosphate | Ru5P, no activity with D-ribose-5-phosphate, D-arabinose, D-glucose-6-phosphate, D-mannose-6-phosphate, alpha-D-glucose-1-phosphate and D-glucosamine-6-phosphate | r | |
| D-Arabinose 5-phosphate | A5P | Escherichia coli | D-Ribulose 5-phosphate | Ru5P, no activity with D-ribose-5-phosphate, D-arabinose, D-glucose-6-phosphate, D-mannose-6-phosphate, alpha-D-glucose-1-phosphate and D-glucosamine-6-phosphate | r | |
| D-Arabinose 5-phosphate | - |
Escherichia coli CFT073 | D-Ribulose 5-phosphate | no activity with D-ribose-5-phosphate, D-arabinose, D-glucose-6-phosphate, D-mannose-6-phosphate, alpha-D-glucose-1-phosphate and D-glucosamine-6-phosphate | r | |
| D-Arabinose 5-phosphate | - |
Escherichia coli CFT073 | D-Ribulose 5-phosphate | Ru5P, no activity with D-ribose-5-phosphate, D-arabinose, D-glucose-6-phosphate, D-mannose-6-phosphate, alpha-D-glucose-1-phosphate and D-glucosamine-6-phosphate | r | |
| D-Arabinose 5-phosphate | A5P | Escherichia coli CFT073 | D-Ribulose 5-phosphate | Ru5P, no activity with D-ribose-5-phosphate, D-arabinose, D-glucose-6-phosphate, D-mannose-6-phosphate, alpha-D-glucose-1-phosphate and D-glucosamine-6-phosphate | r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 x 133909, MALDI-MS | Escherichia coli |
| tetramer | 4 x 35000, SDS-PAGE, MALDI-MS | Escherichia coli |
| tetramer | 4 x 35100, MALDI-MS | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| G-API | D-glucitol phosphotranferase operon related API | Escherichia coli |
| K-API | capsular K-antigen related API | Escherichia coli |
| KpsF | - |
Escherichia coli |
| KPSF protein | - |
Escherichia coli |
| L-API | LPS related API | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.75 | - |
- |
Escherichia coli |
| 8.25 | - |
- |
Escherichia coli |
| 8.4 | - |
- |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8.5 | - |
Escherichia coli |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
