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Literature summary for 5.3.1.13 extracted from
- Identification of GutQ from Escherichia coli as a D-arabinose 5-phosphate isomerase (2005), J. Bacteriol., 187, 6936-6942.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 0.01 mM | Escherichia coli |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| GutQ. GutQ is a second copy of D-arabinose 5-phosphate isomerase (API) from the genome of Escherichia coli K-12, with biochemical properties similar to those of KdsD | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | 0.01 mM | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.64 | - |
D-ribulose 5-phosphate | - |
Escherichia coli | |
| 1.2 | - |
D-arabinose 5-phosphate | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | contains 1.4 Zn2+ per subunit | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33909 | - |
4 * 33909, electrospray ionization mass spectrometry | Escherichia coli |
| 133000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Arabinose 5-phosphate | Escherichia coli | the gut operon is inducible. GutQ is not directly involved in D-glucitol catabolism. The conditional mutant TCM15(DELTAgutQDELTAkdsD) is dependent on exogenous D-arabinose 5-phosphate both for lipopolysaccharide synthesis/growth and for upregulation of the gut operon | D-Ribulose 5-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant GutQ | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Arabinose 5-phosphate | - |
Escherichia coli | D-Ribulose 5-phosphate | - |
? | |
| D-Arabinose 5-phosphate | the gut operon is inducible. GutQ is not directly involved in D-glucitol catabolism. The conditional mutant TCM15(DELTAgutQDELTAkdsD) is dependent on exogenous D-arabinose 5-phosphate both for lipopolysaccharide synthesis/growth and for upregulation of the gut operon | Escherichia coli | D-Ribulose 5-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 33909, electrospray ionization mass spectrometry | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GutQ | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 218 | - |
D-arabinose 5-phosphate | - |
Escherichia coli | |
| 242 | - |
D-ribulose 5-phosphate | - |
Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.3 | - |
- |
Escherichia coli |
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Release 2023.1 (January 2023)
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