Literature summary for 5.3.1.12 extracted from

Williams, L.; Nguyen, T.; Li, Y.; Porter, T.N.; Raushel, F.M.
Uronate isomerase: a nonhydrolytic member of the amidohydrolase superfamily with an ambivalent requirement for a divalent metal ion (2006), Biochemistry, 45, 7453-7462.

Search for more literature for 5.3.1.12

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in Escherichia coli	Escherichia coli

Inhibitors

Inhibitors	Comment	Organism	Structure
D-arabinaric acid	competitive inhibitor	Escherichia coli
D-arabinohydroxamate	competitive inhibitor	Escherichia coli
L-Gulonic acid	competitive inhibitor	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.3	-	D-glucuronate	reconstituted with Co2+, 30°C, pH 8	Escherichia coli
0.48	-	D-glucuronate	reconstituted with Mn2+, 30°C, pH 8	Escherichia coli
0.49	-	D-glucuronate	apoenzyme	Escherichia coli
0.5	-	D-glucuronate	reconstituted with Zn2+, 30°C, pH 8	Escherichia coli
0.55	-	D-glucuronate	reconstituted with Cd2+, 30°C, pH 8	Escherichia coli
8.5	-	D-glucuronate	reconstituted with Ni2+, 30°C, pH 8	Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	enzyme contains 1 equiv of zinc per subunit	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P0A8G3	-	-

Purification (Commentary)

Purification (Comment)	Organism
of the wild type and recombinant protein, ammonium sulfate fractionation, gel filtration, and ion exchange chromatography	Escherichia coli

Reaction

Reaction	Comment	Organism	Reaction ID
D-Glucuronate = D-fructuronate	reaction mechanisms in which an active site base abstracts the proton from C2 of D-glucuronate to form a cisenediol intermediate. The conjugate acid then transfers this proton to C1 of the cis-enediol intermediate to form D-fructuronate	Escherichia coli	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-Glucuronate	-	Escherichia coli	D-Fructuronate	-	?

Synonyms

Synonyms	Comment	Organism
Glucuronate isomerase	-	Escherichia coli
Uronate isomerase	URI	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Escherichia coli

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.000013	-	D-arabinaric acid	apo and Zn2+ reconstituted enzyme	Escherichia coli
0.00043	-	L-Gulonic acid	Zn2+ reconstituted enzyme	Escherichia coli
0.00067	-	D-arabinohydroxamate	Zn2+ reconstituted enzyme	Escherichia coli
0.00093	-	D-arabinohydroxamate	apo enzyme	Escherichia coli

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)