Literature summary for 5.3.1.1 extracted from

Zaffagnini, M.; Michelet, L.; Sciabolini, C.; Di Giacinto, N.; Morisse, S.; Marchand, C.H.; Trost, P.; Fermani, S.; Lemaire, S.D.
High-resolution crystal structure and redox properties of chloroplastic triosephosphate isomerase from Chlamydomonas reinhardtii (2014), Mol. Plant, 7, 101-120.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 cells	Chlamydomonas reinhardtii

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using either 65% (v/v) 2-methyl-2,4-pentanediol 0.1 M HEPES, pH 7.5, or 6% (w/v) PEG 6000 0.1 M HEPES, pH 7.0, or 17.5% (w/v) PEG 3350, 0.1 M Bis-Tris, pH 5.5, or 15% (w/v) PEG 3350 0.1 M Bis-Tris, pH 5.0	Chlamydomonas reinhardtii

Inhibitors

Inhibitors	Comment	Organism	Structure
H2O2	the inhibition of the enzyme is negligible when incubated with 0.1 mM H2O2, whereas a 10% inhibition is observed with 1 mM H2O2	Chlamydomonas reinhardtii

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.04	-	D-glyceraldehyde 3-phosphate	in 100 mM triethanolamine (pH 7.4), at 25°C	Chlamydomonas reinhardtii

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
chloroplast	-	Chlamydomonas reinhardtii	9507	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
28249	-	2 * 28249, calculated from amino acid sequence	Chlamydomonas reinhardtii
28266	-	2 * 28266, MALDI-TOF mass spectrometry	Chlamydomonas reinhardtii
58800	-	gel filtration	Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-Glyceraldehyde 3-phosphate	Chlamydomonas reinhardtii	-	Glycerone phosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Chlamydomonas reinhardtii	Q5S7Y5	-	-

Oxidation Stability

Oxidation Stability	Organism
the inhibition of the enzyme is negligible when incubated with 0.1 mM H2O2, whereas a 10% inhibition is observed with 1 mM H2O2	Chlamydomonas reinhardtii

Purification (Commentary)

Purification (Comment)	Organism
Ni2+ HiTrap chelating resin column chromatography	Chlamydomonas reinhardtii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
7193	-	in 100 mM triethanolamine (pH 7.4), at 25°C	Chlamydomonas reinhardtii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-Glyceraldehyde 3-phosphate	-	Chlamydomonas reinhardtii	Glycerone phosphate	-	r

Subunits

Subunits	Comment	Organism
homodimer	2 * 28249, calculated from amino acid sequence	Chlamydomonas reinhardtii
homodimer	2 * 28266, MALDI-TOF mass spectrometry	Chlamydomonas reinhardtii

Synonyms

Synonyms	Comment	Organism
TpI	-	Chlamydomonas reinhardtii
Triosephosphate isomerase	-	Chlamydomonas reinhardtii

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3372	-	D-glyceraldehyde 3-phosphate	in 100 mM triethanolamine (pH 7.4), at 25°C	Chlamydomonas reinhardtii

General Information

General Information	Comment	Organism
metabolism	the chloroplastic isoform participates in the Calvin-Benson cycle	Chlamydomonas reinhardtii

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Release 2023.1 (January 2023)