Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphite | - |
Gallus gallus | |
phosphite | - |
Trypanosoma brucei brucei |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 pLys S cells | Trypanosoma brucei brucei |
expressed in Escherichia coli FB215471(DE3) cells | Gallus gallus |
Protein Variants | Comment | Organism |
---|---|---|
L7RM | the mutant exhibits a 200fold decrease in kcat/Km for isomerization of D-glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. The mutant exhibits a 25fold decrease in kcat/Km for deprotonation of glycolaldehyde catalyzed by free enzyme. The mutation has little effect on the observed and intrinsic phosphodianion binding energy and only a modest effect on phosphite dianion activation of the enzyme | Gallus gallus |
P168A | the mutation results in 50fold decrease in kcat/Km for deprotonation of glycolaldehyde catalyzed by free enzyme | Trypanosoma brucei brucei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Phosphoglycolate | competitive inhibition | Gallus gallus | |
2-Phosphoglycolate | competitive inhibition | Trypanosoma brucei brucei | |
arsenate | competitive inhibition | Gallus gallus | |
arsenate | competitive inhibition | Trypanosoma brucei brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.091 | - |
D-glyceraldehyde 3-phosphate | mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C | Trypanosoma brucei brucei | |
0.25 | - |
D-glyceraldehyde 3-phosphate | wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C | Trypanosoma brucei brucei | |
0.27 | - |
D-glyceraldehyde 3-phosphate | mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C | Gallus gallus | |
0.29 | - |
D-glyceraldehyde 3-phosphate | wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C | Gallus gallus | |
0.49 | - |
dihydroxyacetone phosphate | mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C | Trypanosoma brucei brucei | |
0.59 | - |
dihydroxyacetone phosphate | wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C | Gallus gallus | |
0.7 | - |
dihydroxyacetone phosphate | wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C | Trypanosoma brucei brucei | |
4 | - |
dihydroxyacetone phosphate | mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C | Gallus gallus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate | Gallus gallus | - |
dihydroxyacetone phosphate | - |
r | |
D-glyceraldehyde 3-phosphate | Trypanosoma brucei brucei | - |
dihydroxyacetone phosphate | - |
r | |
dihydroxyacetone phosphate | Gallus gallus | - |
D-glyceraldehyde 3-phosphate | - |
r | |
dihydroxyacetone phosphate | Trypanosoma brucei brucei | - |
D-glyceraldehyde 3-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
- |
- |
Trypanosoma brucei brucei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
CM Sepharose column chromatography | Trypanosoma brucei brucei |
DEAE-Sepharose column chromatography | Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Gallus gallus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate | - |
Gallus gallus | dihydroxyacetone phosphate | - |
r | |
D-glyceraldehyde 3-phosphate | - |
Trypanosoma brucei brucei | dihydroxyacetone phosphate | - |
r | |
dihydroxyacetone phosphate | - |
Gallus gallus | D-glyceraldehyde 3-phosphate | - |
r | |
dihydroxyacetone phosphate | - |
Trypanosoma brucei brucei | D-glyceraldehyde 3-phosphate | - |
r | |
glycolaldehyde | - |
Gallus gallus | ? | - |
? | |
glycolaldehyde | - |
Trypanosoma brucei brucei | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TIM | - |
Gallus gallus |
TIM | - |
Trypanosoma brucei brucei |
Triosephosphate isomerase | - |
Gallus gallus |
Triosephosphate isomerase | - |
Trypanosoma brucei brucei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.5 | - |
dihydroxyacetone phosphate | mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C | Trypanosoma brucei brucei | |
8 | - |
dihydroxyacetone phosphate | mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C | Gallus gallus | |
16 | - |
D-glyceraldehyde 3-phosphate | mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C | Gallus gallus | |
24 | - |
D-glyceraldehyde 3-phosphate | mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C | Trypanosoma brucei brucei | |
300 | - |
dihydroxyacetone phosphate | wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C | Trypanosoma brucei brucei | |
340 | - |
dihydroxyacetone phosphate | wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C | Gallus gallus | |
2100 | - |
D-glyceraldehyde 3-phosphate | wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C | Trypanosoma brucei brucei | |
3200 | - |
D-glyceraldehyde 3-phosphate | wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C | Gallus gallus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | - |
2-Phosphoglycolate | wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C | Gallus gallus | |
0.055 | - |
2-Phosphoglycolate | wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C | Trypanosoma brucei brucei | |
0.14 | - |
2-Phosphoglycolate | mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C | Trypanosoma brucei brucei | |
2.3 | - |
2-Phosphoglycolate | mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C | Gallus gallus | |
3.8 | - |
arsenate | mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C | Gallus gallus | |
4.6 | - |
arsenate | wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C | Trypanosoma brucei brucei | |
9.6 | - |
arsenate | wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C | Gallus gallus | |
10 | - |
arsenate | mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C | Trypanosoma brucei brucei |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
dihydroxyacetone phosphate | mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C | Gallus gallus | |
13 | - |
dihydroxyacetone phosphate | mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C | Trypanosoma brucei brucei | |
59 | - |
D-glyceraldehyde 3-phosphate | mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C | Gallus gallus | |
260 | - |
D-glyceraldehyde 3-phosphate | mutant enzyme L7RM, in 30 mM triethanolamine buffer at pH 7.5 and 25°C | Trypanosoma brucei brucei | |
430 | - |
dihydroxyacetone phosphate | wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C | Trypanosoma brucei brucei | |
580 | - |
dihydroxyacetone phosphate | wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C | Gallus gallus | |
8400 | - |
D-glyceraldehyde 3-phosphate | wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C | Trypanosoma brucei brucei | |
11000 | - |
D-glyceraldehyde 3-phosphate | wild type enzyme, in 30 mM triethanolamine buffer at pH 7.5 and 25°C | Gallus gallus |