Cloned (Comment) | Organism |
---|---|
expression of TIM in Escherichia coli | Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, 200 mg/ml protein with ligand in a ratio of 5:1 in 50 mM Tris-HCl, 50 mM NaCl, and 1 mM EDTA, pH 6.8, 4°C, is mixed with 40% w/v PEG 4000 as precipitant, X-ray diffraction structure determination and analysis | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Glyceraldehyde 3-phosphate | Saccharomyces cerevisiae | - |
Glycerone phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P00942 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant TIM from Escherichia coli | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Glyceraldehyde 3-phosphate | - |
Saccharomyces cerevisiae | Glycerone phosphate | - |
? | |
D-Glyceraldehyde 3-phosphate | substrate binding perturbs residues Asn10, His95, Ser100, Glu129-Glu133, Val167-Ala186, Asn213, and Leu230-Leu126 that either are near the ligand or have direct contact with the ligand | Saccharomyces cerevisiae | Glycerone phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | determination of conformational dynamics of the enzyme, in solid state and in solution, with or without bound ligand, by NMR analysis, detailed overview | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
TIM | - |
Saccharomyces cerevisiae |
Triosephosphate isomerase | - |
Saccharomyces cerevisiae |