Literature summary for 5.3.1.1 extracted from

Banerjee, M.; Balaram, H.; Balaram, P.
Structural effects of a dimer interface mutation on catalytic activity of triosephosphate isomerase. The role of conserved residues and complementary mutations (2009), FEBS J., 276, 4169-4183.

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Application

Application	Comment	Organism
additional information	the glycolytic enzyme triosephosphate isomerase occupies a central position in the development of structural and mechanistic enzymology	Plasmodium falciparum

Protein Variants

Protein Variants	Comment	Organism
W11F/W168F/Y74W	site-directed mutagenesis, template is the available crystal structure of the enzyme from Giardia lamblia, which contains a Trp residue at position 47. The mutant dissociates at low protein concentrations, and exhibits considerably reduced stability in the presence of denaturants, urea and guanidinium chloride, and it shows approximately 20fold reduction in kcat at low protein concetrations compared to the wild-type enzyme, but the mutant mutant shows an enhancement of activity of 21.9fold at higher concentration range	Plasmodium falciparum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
27000	-	2 * 27000, gel filtration and SDS-PAGE, the active site of triosephosphate isomerase lies very close to the subunit interface, a network of key interactions spans the interacting subunits	Plasmodium falciparum
54000	-	gel filtration	Plasmodium falciparum

Organism

Organism	UniProt	Comment	Textmining
Plasmodium falciparum	Q07412	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	importance of conserved residues in the vicinity of the active site that serve to position the functional K12 residue. A network of key interactions spans the interacting subunits	Plasmodium falciparum	?	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 27000, gel filtration and SDS-PAGE, the active site of triosephosphate isomerase lies very close to the subunit interface, a network of key interactions spans the interacting subunits	Plasmodium falciparum

Synonyms

Synonyms	Comment	Organism
TIM	-	Plasmodium falciparum
Triosephosphate isomerase	-	Plasmodium falciparum

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Release 2023.1 (January 2023)