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Literature summary for 5.3.1.1 extracted from
- Hydron transfer catalyzed by triosephosphate isomerase. Products of the direct and phosphite-activated isomerization of [1-(13)C]-glycolaldehyde in D2O (2009), Biochemistry, 48, 5769-5778.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of the wild-type enzyme in TIM-deficient Escherichia coli strain DF502 | Gallus gallus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Glyceraldehyde 3-phosphate | Gallus gallus | - |
Glycerone phosphate | - |
r |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type enzyme from TIM-deficient Escherichia coli strain DF502 by anion exchange chromatography | Gallus gallus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| skeletal muscle | - |
Gallus gallus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
quantitative TIM-catalyzed isomerization of D-glyceraldehyde 3-phosphate to form glycerone phosphate is coupled to the oxidation of NADH using glycerol 3-phosphate dehydrogenase | Gallus gallus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Glyceraldehyde 3-phosphate | - |
Gallus gallus | Glycerone phosphate | - |
r | |
| D-Glyceraldehyde 3-phosphate | stereospecific reaction via an enzyme-bound enediol(ate) intermediate, proton transfer from D-glyceraldehyde 3-phosphate to the carboxylate side chain of TIM Glu165, irreversible labeling with deuterium , overview | Gallus gallus | Glycerone phosphate | - |
r | |
| glycolaldehyde | TIM-catalyzed reactions of glycolaldehyde are activated by phosphite dianion, kinetics, overview | Gallus gallus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TIM | - |
Gallus gallus |
| Triosephosphate isomerase | - |
Gallus gallus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Gallus gallus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Gallus gallus |
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Release 2023.1 (January 2023)
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