Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-carboxyethyl methanethiosulfonate | modifies four Cys per subunit of dimeric protein and induces 97% of inactivation. Inactivation does not affect secondary structure nor induce dimer dissociation. Cys modification decreases thermal stability of the enzyme | Giardia intestinalis | |
5,5'-dithio-bis(2-nitrobenzoic acid) | derivatizes four of the five Cys per subunit of dimeric protein, resulting in inactivation and dissociation of the dimer to stable monomers | Giardia intestinalis | |
methyl methanethiosulfonate | derivatizes three of the five Cys per subunit of dimeric protein and induces 50% of inactivation. Inactivation does not affect secondary structure nor induce dimer dissociation. Cys modification decreases thermal stability of the enzyme | Giardia intestinalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
D-glyceraldehyde 3-phosphate | - |
Giardia intestinalis | |
0.97 | - |
D-glyceraldehyde 3-phosphate | presence of methyl methanethiosulfonate | Giardia intestinalis | |
8.6 | - |
D-glyceraldehyde 3-phosphate | presence of 2-carboxyethyl methanethiosulfonate | Giardia intestinalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Giardia intestinalis | P36186 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glyceraldehyde 3-phosphate | - |
Giardia intestinalis | dihydroxyacetone phosphate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1666 | - |
D-glyceraldehyde 3-phosphate | presence of 2-carboxyethyl methanethiosulfonate | Giardia intestinalis | |
3166 | - |
D-glyceraldehyde 3-phosphate | presence of methyl methanethiosulfonate | Giardia intestinalis | |
7666 | - |
D-glyceraldehyde 3-phosphate | - |
Giardia intestinalis |