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Literature summary for 5.3.1.1 extracted from

  • Enriquez-Flores, S.; Rodriguez-Romero, A.; Hernandez-Alcantara, G.; De la Mora-De la Mora, I.; Gutierrez-Castrellon, P.; Carvajal, K.; Lopez-Velazquez, G.; Reyes-Vivas, H.
    Species-specific inhibition of Giardia lamblia triosephosphate isomerase by localized perturbation of the homodimer (2008), Mol. Biochem. Parasitol., 157, 179-186.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-carboxyethyl methanethiosulfonate modifies four Cys per subunit of dimeric protein and induces 97% of inactivation. Inactivation does not affect secondary structure nor induce dimer dissociation. Cys modification decreases thermal stability of the enzyme Giardia intestinalis
5,5'-dithio-bis(2-nitrobenzoic acid) derivatizes four of the five Cys per subunit of dimeric protein, resulting in inactivation and dissociation of the dimer to stable monomers Giardia intestinalis
methyl methanethiosulfonate derivatizes three of the five Cys per subunit of dimeric protein and induces 50% of inactivation. Inactivation does not affect secondary structure nor induce dimer dissociation. Cys modification decreases thermal stability of the enzyme Giardia intestinalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
D-glyceraldehyde 3-phosphate
-
Giardia intestinalis
0.97
-
D-glyceraldehyde 3-phosphate presence of methyl methanethiosulfonate Giardia intestinalis
8.6
-
D-glyceraldehyde 3-phosphate presence of 2-carboxyethyl methanethiosulfonate Giardia intestinalis

Organism

Organism UniProt Comment Textmining
Giardia intestinalis P36186
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate
-
Giardia intestinalis dihydroxyacetone phosphate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1666
-
D-glyceraldehyde 3-phosphate presence of 2-carboxyethyl methanethiosulfonate Giardia intestinalis
3166
-
D-glyceraldehyde 3-phosphate presence of methyl methanethiosulfonate Giardia intestinalis
7666
-
D-glyceraldehyde 3-phosphate
-
Giardia intestinalis