Cloned (Comment) | Organism |
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- |
Homo sapiens |
Crystallization (Comment) | Organism |
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mutant E104D, at 1.85 A resolution. Mutant structure is similar to wild-type, mutant residue E104A is part of a conserved cluster of 10 residues, 5 from each subunit. This cluster forms a cavity that possesses an elaborate conserved network of buried water molecules that bridge the two subunits. In the E104D mutant, a disruption of contacts of the amino acid side chains in the conserved cluster leads to a perturbation of the water network in which the water-protein and water-water interactions that join the two monomers are significantly weakened and diminished | Homo sapiens |
Protein Variants | Comment | Organism |
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E104D | mutation involved in human triosephosphate isomerase defiecency autosomal disease. Mutant exhibits normal catalytic activity but shows impairments in the formation of active dimers and low thermostability. E104A is part of a conserved cluster of 10 residues, 5 from each subunit. This cluster forms a cavity that possesses an elaborate conserved network of buried water molecules that bridge the two subunits. In the E104D mutant, a disruption of contacts of the amino acid side chains in the conserved cluster leads to a perturbation of the water network in which the water-protein and water-water interactions that join the two monomers are significantly weakened and diminished | Homo sapiens |
Organism | UniProt | Comment | Textmining |
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Homo sapiens | P60174 | - |
- |
Purification (Comment) | Organism |
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recombinant mutant E104D | Homo sapiens |