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Literature summary for 5.3.1.1 extracted from

  • Alahuhta, M.; Casteleijn, M.G.; Neubauer, P.; Wierenga, R.K.
    Structural studies show that the A178L mutation in the C-terminal hinge of the catalytic loop-6 of triosephosphate isomerase (TIM) induces a closed-like conformation in dimeric and monomeric TIM (2008), Acta Crystallogr. Sect. D, 64, 178-188.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant A178L, unliganded and in complex with 2-phosphoglycolate, to 2.2 A and 1.89 A, respectively. Monomeric mutant mlA178L, unliganded and in complex with 2-phosphoglycolate, to 2.3 A and 1.18 A, respectively. Mutation A178L favors the closed conformation of the C-terminal hinge region Trypanosoma brucei

Protein Variants

Protein Variants Comment Organism
A178L decrease in catalytic efficiency. Crystallization data reveal a more disordered loop-6 in the structure of unliganded A178L. Liganded structures show minimal differences to wild-type Trypanosoma brucei

Inhibitors

Inhibitors Comment Organism Structure
2-Phosphoglycolate
-
Trypanosoma brucei
AsO43-
-
Trypanosoma brucei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.26
-
D-glyceraldehyde 3-phosphate wild-type, pH 7.6, 25°C Trypanosoma brucei
0.61
-
D-glyceraldehyde 3-phosphate mutant A178L, pH 7.6, 25°C Trypanosoma brucei
0.9
-
dihydroxyacetone phosphate wild-type, pH 7.6, 25°C Trypanosoma brucei
1.4
-
dihydroxyacetone phosphate mutant A178L, pH 7.6, 25°C Trypanosoma brucei
3.3
-
D-glyceraldehyde 3-phosphate monomeric mutant A178L, pH 7.6, 25°C Trypanosoma brucei
3.8
-
D-glyceraldehyde 3-phosphate monomeric wild-type, pH 7.6, 25°C Trypanosoma brucei
7.6
-
dihydroxyacetone phosphate monomeric wild-type, pH 7.6, 25°C Trypanosoma brucei
9.3
-
dihydroxyacetone phosphate monomeric mutant A178L, pH 7.6, 25°C Trypanosoma brucei

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei P04789
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate
-
Trypanosoma brucei dihydroxyacetone phosphate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.06
-
dihydroxyacetone phosphate monomeric mutant A178L, pH 7.6, 25°C Trypanosoma brucei
0.25
-
D-glyceraldehyde 3-phosphate monomeric mutant A178L, pH 7.6, 25°C Trypanosoma brucei
0.8
-
dihydroxyacetone phosphate monomeric wild-type, pH 7.6, 25°C Trypanosoma brucei
2.2
-
D-glyceraldehyde 3-phosphate monomeric wild-type, pH 7.6, 25°C Trypanosoma brucei
500
-
dihydroxyacetone phosphate mutant A178L, pH 7.6, 25°C Trypanosoma brucei
800
-
dihydroxyacetone phosphate wild-type, pH 7.6, 25°C Trypanosoma brucei
4400
-
D-glyceraldehyde 3-phosphate wild-type, pH 7.6, 25°C Trypanosoma brucei
5300
-
D-glyceraldehyde 3-phosphate mutant A178L, pH 7.6, 25°C Trypanosoma brucei

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.05
-
2-Phosphoglycolate wild-type, pH 7.6, 25°C Trypanosoma brucei
0.17
-
2-Phosphoglycolate monomeric mutant A178L, pH 7.6, 25°C Trypanosoma brucei
0.39
-
2-Phosphoglycolate mutant A178L, pH 7.6, 25°C Trypanosoma brucei
4.9
-
AsO43- wild-type, pH 7.6, 25°C Trypanosoma brucei
5.1
-
AsO43- monomeric wild-type, pH 7.6, 25°C Trypanosoma brucei
7.8
-
AsO43- mutant A178L, pH 7.6, 25°C Trypanosoma brucei
17.9
-
AsO43- monomeric mutant A178L, pH 7.6, 25°C Trypanosoma brucei