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Literature summary for 5.3.1.1 extracted from

  • Xiang, J.; Jung, J.Y.; Sampson, N.S.
    Entropy effects on protein hinges: the reaction catalyzed by triosephosphate isomerase (2004), Biochemistry, 43, 11436-11445.
    View publication on PubMed
Search for more literature for 5.3.1.1

Protein Variants

Protein Variants Comment Organism
K174G/T175G/A176G 16.5fold decrease in turnover number for D-glyceraldehyde 3-phosphate, 7.9fold increase in Km-value for D-glyceraldehyde 3-phosphate, 8fold decrease in turnover number for dihydroxyacetone phosphate, 2.6fold increase in Ki-value for arsenate, 10.4fold increase in Ki-value for 2-phosphoglycolate Gallus gallus
V167G/W168G 17.9fold decrease in turnover number for D-glyceraldehyde 3-phosphate, 2.6fold increase in Km-value for D-glyceraldehyde 3-phosphate, 8.6fold decrease in turnover number for dihydroxyacetone phosphate, 2.7fold increase in Ki-value for arsenate, 8.1fold increase in Ki-value for 2-phosphoglycolate Gallus gallus
V167G/W168G/K174G/T175G/A176G 2529fold decrease in turnover number for D-glyceraldehyde 3-phosphate, 8.5fold increase in Km-value for D-glyceraldehyde 3-phosphate, 1720fold decrease in turnover number for dihydroxyacetone phosphate, 2.8fold increase in Ki-value for arsenate, 12.4fold increase in Ki-value for 2-phosphoglycolate Gallus gallus

Inhibitors

Inhibitors Comment Organism Structure
2-Phosphoglycolate
-
 Gallus gallus
arsenate
-
 Gallus gallus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.47
-
 D-glyceraldehyde 3-phosphate 30°C, pH 7.6, wild-type enzyme Gallus gallus
0.97
-
 dihydroxyacetone phosphate 30°C, pH 7.6, wild-type enzyme Gallus gallus
1.2
-
 D-glyceraldehyde 3-phosphate 30°C, pH 7.6, mutant enzyme V167G/W168G Gallus gallus
3.7
-
 D-glyceraldehyde 3-phosphate 30°C, pH 7.6, mutant enzyme K174G/T175G/A176G Gallus gallus
4
-
 D-glyceraldehyde 3-phosphate 30°C, pH 7.6, mutant enzyme V167G/W168G/K174G/T175G/A176G Gallus gallus
11
-
 dihydroxyacetone phosphate 30°C, pH 7.6, mutant enzyme V167G/W168G Gallus gallus
15
-
 dihydroxyacetone phosphate 30°C, pH 7.6, mutant enzyme K174G/T175G/A176G Gallus gallus
18
-
 dihydroxyacetone phosphate 30°C, pH 7.6, mutant enzyme V167G/W168G/K174G/T175G/A176G Gallus gallus

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glyceraldehyde 3-phosphate
-
 Gallus gallus dihydroxyacetone phosphate
-
 r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.25
-
 dihydroxyacetone phosphate 30°C, pH 7.6, mutant enzyme V167G/W168G/K174G/T175G/A176G Gallus gallus
1.7
-
 D-glyceraldehyde 3-phosphate 30°C, pH 7.6, mutant enzyme V167G/W168G/K174G/T175G/A176G Gallus gallus
50
-
 dihydroxyacetone phosphate 30°C, pH 7.6, mutant enzyme V167G/W168G Gallus gallus
54
-
 dihydroxyacetone phosphate 30°C, pH 7.6, mutant enzyme K174G/T175G/A176G Gallus gallus
240
-
 D-glyceraldehyde 3-phosphate 30°C, pH 7.6, mutant enzyme V167G/W168G Gallus gallus
261
-
 D-glyceraldehyde 3-phosphate 30°C, pH 7.6, mutant enzyme K174G/T175G/A176G Gallus gallus
430
-
 dihydroxyacetone phosphate 30°C, pH 7.6, wild-type enzyme Gallus gallus
4300
-
 D-glyceraldehyde 3-phosphate 30°C, pH 7.6, wild-type enzyme Gallus gallus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
11
-
 arsenate 30°C, pH 7.6, wild-type enzyme Gallus gallus
29
-
 arsenate 30°C, pH 7.6, mutant enzyme K174G/T175G/A176G Gallus gallus
30
-
 arsenate 30°C, pH 7.6, mutant enzyme V167G/W168G Gallus gallus
31
-
 arsenate 30°C, pH 7.6, mutant enzyme V167G/W168G/K174G/T175G/A176G Gallus gallus