Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells | Trypanosoma cruzi |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | Par45 is mainly concentrated in the nucleus | Trypanosoma cruzi | 5634 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45400 | - |
x * 45400, calculated from amino acid sequence | Trypanosoma cruzi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | B8XP93 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography and Superdex 75 gel filtration | Trypanosoma cruzi |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
amastigote | - |
Trypanosoma cruzi | - |
epimastigote | - |
Trypanosoma cruzi | - |
trypomastigote | - |
Trypanosoma cruzi | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Par45 does not accelerate the cis/trans interconversion of acidic substrates containing Glu-Pro bonds | Trypanosoma cruzi | ? | - |
? | |
N-succinyl-Ala-Ala-(cis)-Pro-Phe-4-nitroanilide | 6.9% activity compared to N-succinyl-Ala-Arg-(cis)-Pro-Phe-4-nitroanilide | Trypanosoma cruzi | N-succinyl-Ala-Ala-(trans)-Pro-Phe-4-nitroanilide | - |
? | |
N-succinyl-Ala-Arg-(cis)-Pro-Phe-4-nitroanilide | 100% activity, Par45 shows a strong preference for a substrate with the basic Arg residue preceding Pro | Trypanosoma cruzi | N-succinyl-Ala-Arg-(trans)-Pro-Phe-4-nitroanilide | - |
? | |
N-succinyl-Ala-Glu-(cis)-Pro-Phe-4-nitroanilide | 7.6% activity compared to N-succinyl-Ala-Arg-(cis)-Pro-Phe-4-nitroanilide | Trypanosoma cruzi | N-succinyl-Ala-Glu-(trans)-Pro-Phe-4-nitroanilide | - |
? | |
N-succinyl-Ala-Leu-(cis)-Pro-Phe-4-nitroanilide | 26.7% activity compared to N-succinyl-Ala-Arg-(cis)-Pro-Phe-4-nitroanilide | Trypanosoma cruzi | N-succinyl-Ala-Leu-(trans)-Pro-Phe-4-nitroanilide | - |
? | |
N-succinyl-Ala-Phe-(cis)-Pro-Phe-4-nitroanilide | 4.3% activity compared to N-succinyl-Ala-Arg-(cis)-Pro-Phe-4-nitroanilide | Trypanosoma cruzi | N-succinyl-Ala-Phe-(trans)-Pro-Phe-4-nitroanilide | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45400, calculated from amino acid sequence | Trypanosoma cruzi |
Synonyms | Comment | Organism |
---|---|---|
Par45 | - |
Trypanosoma cruzi |
peptidyl-prolyl cis/trans isomerase | - |
Trypanosoma cruzi |
PPIase | - |
Trypanosoma cruzi |
General Information | Comment | Organism |
---|---|---|
physiological function | Par45 is a phosphorylation-independent parvulin required for normal cell proliferation in a unicellular eukaryotic cell | Trypanosoma cruzi |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.2 | - |
N-succinyl-Ala-Phe-(cis)-Pro-Phe-4-nitroanilide | at 10 °C in 35 mM sodium HEPES buffer, pH 7.8 | Trypanosoma cruzi | |
6.7 | - |
N-succinyl-Ala-Ala-(cis)-Pro-Phe-4-nitroanilide | at 10 °C in 35 mM sodium HEPES buffer, pH 7.8 | Trypanosoma cruzi | |
7.4 | - |
N-succinyl-Ala-Glu-(cis)-Pro-Phe-4-nitroanilide | at 10 °C in 35 mM sodium HEPES buffer, pH 7.8 | Trypanosoma cruzi | |
25.9 | - |
N-succinyl-Ala-Leu-(cis)-Pro-Phe-4-nitroanilide | at 10 °C in 35 mM sodium HEPES buffer, pH 7.8 | Trypanosoma cruzi | |
97.1 | - |
N-succinyl-Ala-Arg-(cis)-Pro-Phe-4-nitroanilide | at 10 °C in 35 mM sodium HEPES buffer, pH 7.8 | Trypanosoma cruzi |