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Literature summary for 5.2.1.5 extracted from
- Purification, immobilization and characterization of linoleic acid isomerase on modified palygorskite (2011), Bioprocess Biosyst. Eng., 34, 757-765.
General Stability
| General Stability | Organism |
|---|---|
| the immobilized enzyme has high operational stability and retains high enzymatic activity after seven cycles of reuse at 37°C. After incubation for 3 h, the immobilized linoleic acid isomerase retains 85% of its initial activity, and then its activity is slowly decreased to 45% within 24 h | Lactobacillus delbrueckii subsp. bulgaricus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0619 | - |
9-cis,12-cis-linoleic acid | pH 6.5, 30°C | Lactobacillus delbrueckii subsp. bulgaricus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41000 | - |
x * 41000, SDS-PAGE | Lactobacillus delbrueckii subsp. bulgaricus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactobacillus delbrueckii subsp. bulgaricus | - |
- |
- |
| Lactobacillus delbrueckii subsp. bulgaricus 1.1480 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, DEAE-Sepharose column chromatography and Sephadex G-100 gel filtration | Lactobacillus delbrueckii subsp. bulgaricus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.8 | - |
crude extract, at pH 6.5, 30°C | Lactobacillus delbrueckii subsp. bulgaricus |
| 353.5 | - |
after 93.3fold purification, at pH 6.5, 30°C | Lactobacillus delbrueckii subsp. bulgaricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 9-cis,12-cis-linoleic acid | - |
Lactobacillus delbrueckii subsp. bulgaricus | 9-cis,11-trans-linoleic acid | - |
? | |
| 9-cis,12-cis-linoleic acid | - |
Lactobacillus delbrueckii subsp. bulgaricus 1.1480 | 9-cis,11-trans-linoleic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 41000, SDS-PAGE | Lactobacillus delbrueckii subsp. bulgaricus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| C12 isomerase | - |
Lactobacillus delbrueckii subsp. bulgaricus |
| LA isomerase | - |
Lactobacillus delbrueckii subsp. bulgaricus |
| Linoleic acid isomerase | - |
Lactobacillus delbrueckii subsp. bulgaricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
free enzyme | Lactobacillus delbrueckii subsp. bulgaricus |
| 35 | - |
immobilized enzyme | Lactobacillus delbrueckii subsp. bulgaricus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 40 | - |
Lactobacillus delbrueckii subsp. bulgaricus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | the immobilized enzyme is more stable than the free enzyme at 30-60°C, more than 85% of its initial activity is retained after incubation for 3 h. The residual activity of the free and immobilized LA isomerase is 100% at 20°C. The free and immobilized enzymes are highly stable below 30°C. However, the activity of the free enzyme rapidly decreases with the increased temperature, and the immobilized enzyme retains its activity above 30°C. The immobilized enzyme has 81% activity at 50°C, whereas the free enzyme loses 51% of its activity | Lactobacillus delbrueckii subsp. bulgaricus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
free enzyme | Lactobacillus delbrueckii subsp. bulgaricus |
| 7 | - |
immobilized enzyme | Lactobacillus delbrueckii subsp. bulgaricus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8 | the residual activity of the free and immobilized linoleic acid isomerase is 100% at pH 6.5 and 7.0, respectively. The immobilized linoleic acid isomerase remains more than 60% of its initial activity in the pH range of 5.5-8.0 and more than 80% of its initial activity in the pH range of 6.0-8.0, while the free linoleic acid isomerase remains more than 60% of its initial activity in the pH range of 5.5-7.0 and more than 80% of its initial activity in the pH range of 6.0-7.0 | Lactobacillus delbrueckii subsp. bulgaricus |
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