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Literature summary for 5.2.1.4 extracted from

  • Liu, T.T.; Xu, Y.; Liu, H.; Luo, S.; Yin, Y.J.; Liu, S.J.; Zhou, N.Y.
    Functional characterization of a gene cluster involved in gentisate catabolism in Rhodococcus sp. strain NCIMB 12038 (2010), Appl. Microbiol. Biotechnol., 90, 671-678.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3) pLysS cells Rhodococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
gel filtration Rhodococcus sp.
30000
-
1 * 30000, SDS-PAGE Rhodococcus sp.

Organism

Organism UniProt Comment Textmining
Rhodococcus sp.
-
-
-
Rhodococcus sp. NCIMB 12038
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Rhodococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Maleylpyruvate
-
Rhodococcus sp. Fumarylpyruvate
-
?
Maleylpyruvate
-
Rhodococcus sp. NCIMB 12038 Fumarylpyruvate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 30000, SDS-PAGE Rhodococcus sp.

Synonyms

Synonyms Comment Organism
mycothiol-dependent maleylpyruvate isomerase
-
Rhodococcus sp.
NarL
-
Rhodococcus sp.

Cofactor

Cofactor Comment Organism Structure
mycothiol dependent on Rhodococcus sp.