Literature summary for 5.2.1.4 extracted from

Wang, R.; Yin, Y.J.; Wang, F.; Li, M.; Feng, J.; Zhang, H.M.; Zhang, J.P.; Liu, S.J.; Chang, W.R.
Crystal structures and site-directed mutagenesis of a mycothiol-dependent enzyme reveal a novel folding and molecular basis for mycothiol-mediated maleylpyruvate isomerization (2007), J. Biol. Chem., 282, 16288-16294.

Search for more literature for 5.2.1.4

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Corynebacterium glutamicum

Crystallization (Commentary)

Crystallization (Comment)	Organism
of the wild type and one mutated enzyme (H52A), enzyme contains two domains	Corynebacterium glutamicum

Protein Variants

Protein Variants	Comment	Organism
C61A	site directed mutagenesis	Corynebacterium glutamicum
D151A	site directed mutagenesis, completely inactive	Corynebacterium glutamicum
E144A	site directed mutagenesis, completely inactive	Corynebacterium glutamicum
E85A	site directed mutagenesis	Corynebacterium glutamicum
H148A	site directed mutagenesis, completely inactive	Corynebacterium glutamicum
H48A	site directed mutagenesis	Corynebacterium glutamicum
H52A	site directed mutagenesis, completely inactive	Corynebacterium glutamicum
N56A	site directed mutagenesis, completely inactive	Corynebacterium glutamicum
R141A	site directed mutagenesis, completely inactive	Corynebacterium glutamicum
R222A	site directed mutagenesis, completely inactive	Corynebacterium glutamicum
R82A	site directed mutagenesis, partially inactive	Corynebacterium glutamicum
S78A	site directed mutagenesis, almost completely inactive	Corynebacterium glutamicum
W44A	site directed mutagenesis, completely inactive	Corynebacterium glutamicum
Y76A	site directed mutagenesis, completely inactive	Corynebacterium glutamicum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	probably required for activity	Corynebacterium glutamicum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
28000	-	SDS-PAGE, gel filtration	Corynebacterium glutamicum

Organism

Organism	UniProt	Comment	Textmining
Corynebacterium glutamicum	Q8NLC1	wild type	-

Purification (Commentary)

Purification (Comment)	Organism
of the recombinant wild type and mutant enzymes	Corynebacterium glutamicum

Storage Stability

Storage Stability	Organism
at -80°C	Corynebacterium glutamicum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3-maleylpyruvate +	-	Corynebacterium glutamicum	3-fumarylpyruvate	-	?

Synonyms

Synonyms	Comment	Organism
3-maleylpyruvate cis-trans-isomerase	-	Corynebacterium glutamicum
maleylpyruvate isomerase	-	Corynebacterium glutamicum
MDMPI	mycothiol dependent maleylpyruvate isomerase	Corynebacterium glutamicum

Cofactor

Cofactor	Comment	Organism	Structure
additional information	MSH, mycothiol, 1D-myo-inosityl-1-(N-acetyl-L-cysteinyl)amido-2-deoxy-a-D-glucopyranoside	Corynebacterium glutamicum

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Release 2023.1 (January 2023)