Activating Compound | Comment | Organism | Structure |
---|---|---|---|
GSH | required as coenzyme | Vibrio sp. | |
GSH | other thiols are inactive | Vibrio sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GSSG | - |
Vibrio sp. | |
N-Hexylmaleamic acid | noncompetitive towards GSH and maleylacetone | Vibrio sp. | |
NEM | - |
Vibrio sp. | |
S-Glutathioyl-N-hexylmaleamate | competitive towards GSH, uncompetitive towards maleylacetone | Vibrio sp. | |
S-Methylglutathione | - |
Vibrio sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
maleylacetone | - |
Vibrio sp. | |
1.9 | - |
6-Oxo-2(E),4(Z)-heptanedienoic acid | - |
Vibrio sp. | |
2.9 | - |
trans-beta-Acetylacrylate | - |
Vibrio sp. | |
3 | - |
cis-beta-Acetylacrylate | - |
Vibrio sp. | |
3.2 | - |
6-Oxo-2(Z),4(E)-heptanedienoic acid | - |
Vibrio sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16000 | - |
x * 16000, SDS-PAGE after heating the enzyme at 100°C for 10 min in a standard aqueous medium containing 1% SDS and 8M urea | Vibrio sp. |
35000 | - |
gel filtration | Vibrio sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Maleylacetoacetate | Vibrio sp. | one of the enzymes responsible for the oxidative metabolism of aromatic amino acids | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mammalia | - |
- |
- |
Vibrio sp. | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Mammalia | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-Oxo-2(E),4(Z)-heptanedienoic acid | - |
Vibrio sp. | 6-Oxo-2(Z),4(E)-heptanedienoic acid | - |
? | |
cis-beta-Acetylacrylate | - |
Vibrio sp. | trans-beta-Acetylacrylate | - |
? | |
Maleylacetoacetate | - |
Vibrio sp. | Fumarylacetoacetate | - |
? | |
Maleylacetoacetate | one of the enzymes responsible for the oxidative metabolism of aromatic amino acids | Vibrio sp. | ? | - |
? | |
maleylacetone | - |
Vibrio sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35000, SDS-PAGE | Vibrio sp. |
? | x * 16000, SDS-PAGE after heating the enzyme at 100°C for 10 min in a standard aqueous medium containing 1% SDS and 8M urea | Vibrio sp. |