Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.2.1.1 extracted from

  • Liu, X.; Zhao, Q.; Ren, J.; Dong, W.; Wu, Q.; Zhu, D.
    N-terminal truncation of a maleate cis-trans isomerase from Rhodococcus jostii RHA1 results in a highly active enzyme for the biocatalytic production of fumaric acid (2013), J. Mol. Catal. B, 93, 44-50.
No PubMed abstract available

Application

Application Comment Organism
synthesis whole cells of Escherichia coli expressing the enzyme mutant MaiR-D48 catalyze the isomerization of maleic acid to fumaric acid at 1 M substrate concentration showing its potential for industrial use Rhodococcus jostii

Cloned(Commentary)

Cloned (Comment) Organism
gene ro00478, recombinant expression of wild-type and mutants in Escherichia coli Rosetta2 (DE3) pLysS Rhodococcus jostii

Protein Variants

Protein Variants Comment Organism
additional information generation of three catalytically active mutants MaiR-D41/42-304AA, MaiR-D48/49-304AA and MaiR-D52/53-304AA without the hydrophilic and hydrophobic clusters Rhodococcus jostii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1496
-
Maleate pH 8.0, 40°C, recombinant enzyme mutant MaiR-D48/49-304AA Rhodococcus jostii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Maleate Rhodococcus jostii whole cells of recombinant Escherichia coli expressing enzyme mutant MaiR-D48 catalyze the isomerization of maleic acid to fumaric acid at 1 M substrate concentration Fumarate
-
r

Organism

Organism UniProt Comment Textmining
Rhodococcus jostii Q0SJH2 gene ro00478
-

Oxidation Stability

Oxidation Stability Organism
enzyme mutant MaiR-D48 shows high oxidative stability Rhodococcus jostii

Purification (Commentary)

Purification (Comment) Organism
recombinant mutant MaiR-D48/49-304AA 2.7fold from Escherichia coli strain Rosetta 2 (DE3) by ammonium sulfate fractionation, anion exchange chromatography, ultratfiltration, and gel filtration Rhodococcus jostii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
106.8
-
purified enzyme mutant MaiR-D48/49-304AA, pH 8.0, 40°C Rhodococcus jostii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Maleate
-
Rhodococcus jostii Fumarate
-
r
Maleate whole cells of recombinant Escherichia coli expressing enzyme mutant MaiR-D48 catalyze the isomerization of maleic acid to fumaric acid at 1 M substrate concentration Rhodococcus jostii Fumarate
-
r

Synonyms

Synonyms Comment Organism
maleate cis-trans isomerase
-
Rhodococcus jostii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
recombinant mutant MaiR-D48/49-304AA Rhodococcus jostii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 60 activity range, recombinant mutant MaiR-D48/49-304AA Rhodococcus jostii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
purified recombinant mutant MaiR-D48/49-304AA, 30 min, completely stable Rhodococcus jostii
55 60 purified recombinant mutant MaiR-D48/49-304AA, 30 min, over 95% activity at 55°C and about 50% activity at 60°C remaining Rhodococcus jostii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
96.7
-
Maleate pH 8.0, 40°C, recombinant enzyme mutant MaiR-D48/49-304AA Rhodococcus jostii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
recombinant mutant MaiR-D48/49-304AA Rhodococcus jostii

pH Range

pH Minimum pH Maximum Comment Organism
6 9 activity range, recombinant mutant MaiR-D48/49-304AA Rhodococcus jostii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
650
-
Maleate pH 8.0, 40°C, recombinant enzyme mutant MaiR-D48/49-304AA Rhodococcus jostii