Crystallization (Comment) | Organism |
---|---|
im complex with maleate | Nocardia farcinica |
Protein Variants | Comment | Organism |
---|---|---|
C194A | inactive | Nocardia farcinica |
C194S | the mutant displays more than 1000fold reduced turnover compared to the wild type enzyme | Nocardia farcinica |
C76S | the mutant displays more than 1000fold reduced turnover compared to the wild type enzyme | Nocardia farcinica |
Y133F | the mutant shows decreased turnover and Km, but about wild type catalytic efficiency | Nocardia farcinica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bromomaleate | weak inhibitor | Nocardia farcinica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00053 | - |
Maleate | mutant enzyme Y133F, at pH 7.5, temperature not specified in the publication | Nocardia farcinica | |
0.0037 | - |
Maleate | mutant enzyme C76S, at pH 7.5, temperature not specified in the publication | Nocardia farcinica | |
0.0046 | - |
Maleate | wild type enzyme, at pH 7.5, temperature not specified in the publication | Nocardia farcinica | |
0.007 | - |
Maleate | mutant enzyme C194S, at pH 7.5, temperature not specified in the publication | Nocardia farcinica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nocardia farcinica | Q5YXQ1 | - |
- |
Nocardia farcinica IFM 10152 | Q5YXQ1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Maleate | - |
Nocardia farcinica | Fumarate | - |
ir | |
Maleate | - |
Nocardia farcinica IFM 10152 | Fumarate | - |
ir |
Subunits | Comment | Organism |
---|---|---|
dimer | dynamic light scattering | Nocardia farcinica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Nocardia farcinica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00035 | - |
Maleate | mutant enzyme C194S, at pH 7.5, temperature not specified in the publication | Nocardia farcinica | |
0.0019 | - |
Maleate | mutant enzyme C76S, at pH 7.5, temperature not specified in the publication | Nocardia farcinica | |
0.32 | - |
Maleate | mutant enzyme Y133F, at pH 7.5, temperature not specified in the publication | Nocardia farcinica | |
2.8 | - |
Maleate | wild type enzyme, at pH 7.5, temperature not specified in the publication | Nocardia farcinica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Nocardia farcinica |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
bromomaleate | pH 7.5, temperature not specified in the publication | Nocardia farcinica |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
Maleate | mutant enzyme C194S, at pH 7.5, temperature not specified in the publication | Nocardia farcinica | |
0.513 | - |
Maleate | mutant enzyme C76S, at pH 7.5, temperature not specified in the publication | Nocardia farcinica | |
600 | - |
Maleate | mutant enzyme Y133F, at pH 7.5, temperature not specified in the publication | Nocardia farcinica | |
610 | - |
Maleate | wild type enzyme, at pH 7.5, temperature not specified in the publication | Nocardia farcinica |