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Literature summary for 5.2.1.1 extracted from

  • Fisch, F.; Fleites, C.M.; Delenne, M.; Baudendistel, N.; Hauer, B.; Turkenburg, J.P.; Hart, S.; Bruce, N.C.; Grogan, G.
    A covalent succinylcysteine-like intermediate in the enzyme-catalyzed transformation of maleate to fumarate by maleate isomerase (2010), J. Am. Chem. Soc., 132, 11455-11457.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
im complex with maleate Nocardia farcinica

Protein Variants

Protein Variants Comment Organism
C194A inactive Nocardia farcinica
C194S the mutant displays more than 1000fold reduced turnover compared to the wild type enzyme Nocardia farcinica
C76S the mutant displays more than 1000fold reduced turnover compared to the wild type enzyme Nocardia farcinica
Y133F the mutant shows decreased turnover and Km, but about wild type catalytic efficiency Nocardia farcinica

Inhibitors

Inhibitors Comment Organism Structure
bromomaleate weak inhibitor Nocardia farcinica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00053
-
Maleate mutant enzyme Y133F, at pH 7.5, temperature not specified in the publication Nocardia farcinica
0.0037
-
Maleate mutant enzyme C76S, at pH 7.5, temperature not specified in the publication Nocardia farcinica
0.0046
-
Maleate wild type enzyme, at pH 7.5, temperature not specified in the publication Nocardia farcinica
0.007
-
Maleate mutant enzyme C194S, at pH 7.5, temperature not specified in the publication Nocardia farcinica

Organism

Organism UniProt Comment Textmining
Nocardia farcinica Q5YXQ1
-
-
Nocardia farcinica IFM 10152 Q5YXQ1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Maleate
-
Nocardia farcinica Fumarate
-
ir
Maleate
-
Nocardia farcinica IFM 10152 Fumarate
-
ir

Subunits

Subunits Comment Organism
dimer dynamic light scattering Nocardia farcinica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
7.5
-
-
Nocardia farcinica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00035
-
Maleate mutant enzyme C194S, at pH 7.5, temperature not specified in the publication Nocardia farcinica
0.0019
-
Maleate mutant enzyme C76S, at pH 7.5, temperature not specified in the publication Nocardia farcinica
0.32
-
Maleate mutant enzyme Y133F, at pH 7.5, temperature not specified in the publication Nocardia farcinica
2.8
-
Maleate wild type enzyme, at pH 7.5, temperature not specified in the publication Nocardia farcinica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Nocardia farcinica

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.001
-
bromomaleate pH 7.5, temperature not specified in the publication Nocardia farcinica

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.05
-
Maleate mutant enzyme C194S, at pH 7.5, temperature not specified in the publication Nocardia farcinica
0.513
-
Maleate mutant enzyme C76S, at pH 7.5, temperature not specified in the publication Nocardia farcinica
600
-
Maleate mutant enzyme Y133F, at pH 7.5, temperature not specified in the publication Nocardia farcinica
610
-
Maleate wild type enzyme, at pH 7.5, temperature not specified in the publication Nocardia farcinica