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Literature summary for 5.1.99.8 extracted from
- Biosynthesis of pteridines in Escherichia coli. Structural and mechanistic similarity of dihydroneopterin-triphosphate epimerase and dihydroneopterin aldolase (1998), J. Biol. Chem., 273, 17418-17424.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.013 | - |
7,8-dihydroneopterin triphosphate | pH 8.0, 55°C | Escherichia coli |  |
| 0.066 | - |
7,8-dihydromonapterin | pH 8.0, 55°C | Escherichia coli | |
| 0.149 | - |
7,8-dihydroneopterin | pH 8.0, 55°C | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 13988 | - |
8 * 13988, calculated, 8 * 14000, SDS-PAGE | Escherichia coli |
| 14000 | - |
8 * 13988, calculated, 8 * 14000, SDS-PAGE | Escherichia coli |
| 111600 | - |
sedimentation equilibrium analysis | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P0AC19 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.8 | - |
pH 8.0, 55°C | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,8-dihydromonapterin | - |
Escherichia coli | 7,8-dihydroneopterin | - |
? | |
| 7,8-dihydroneopterin | - |
Escherichia coli | 7,8-dihydromonapterin | - |
r | |
| 7,8-dihydroneopterin triphosphate | - |
Escherichia coli | 7,8-dihydromonapterin triphosphate | - |
r | |
| additional information | catalyzes the epimerization of carbon 2' in the triphosphates of dihydroneopterin and dihydromonapterin. The enzyme can also catalyze the cleavage of the position 6 side chain of several pteridine derivatives at a slow rate | Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | 8 * 13988, calculated, 8 * 14000, SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| folX | - |
Escherichia coli |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | a folX deletion mutant has normal growth properties on complete medium as well as on minimal medium | Escherichia coli |
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