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Literature summary for 5.1.99.7 extracted from

  • Pribat, A.; Blaby, I.K.; Lara-Nunez, A.; Gregory, J.F.; de Crecy-Lagard, V.; Hanson, A.D.
    FolX and FolM are essential for tetrahydromonapterin synthesis in Escherichia coli and Pseudomonas aeruginosa (2010), J. Bacteriol., 192, 475-482.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.1.99.7

Cloned(Commentary)

Cloned (Comment) Organism
gene folX, genetic structure and phylogenetic analysis Escherichia coli
gene folX, genetic structure and phylogenetic analysis Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
additional information construction of an Escherichia coli strain that lacks phenylalanine hydroxylase, PhhA, and in which the expression of Pseudomonas aeruginosa PhhA plus the recycling enzyme pterin 4a-carbinolamine dehydratase PhhB, rescues tyrosine auxotrophy. This rescue is abrogated by deleting folX or folM and restored by expressing the deleted gene from a plasmid. The folX deletion selectively eliminates tetrahydromonapterin production, the mutant strain lacks tetrahydromonapterin Escherichia coli
additional information deletion of tyrA (making PhhA the sole source of tyrosine) and folX results in a strain prototrophic for tyrosine, whereas the DELTAtyrA DELTAfolX strain is auxotrophic Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7,8-dihydroneopterin 3'-triphosphate Escherichia coli
-
 7,8-dihydromonapterin 3'-triphosphate
-
 r
7,8-dihydroneopterin 3'-triphosphate Pseudomonas aeruginosa
-
 7,8-dihydromonapterin 3'-triphosphate
-
 r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 gene folX
-
Pseudomonas aeruginosa Q9HYG7 gene folX
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7,8-dihydroneopterin 3'-triphosphate
-
 Escherichia coli 7,8-dihydromonapterin 3'-triphosphate
-
 r
7,8-dihydroneopterin 3'-triphosphate
-
 Pseudomonas aeruginosa 7,8-dihydromonapterin 3'-triphosphate
-
 r

Synonyms

Synonyms Comment Organism
dihydroneopterin triphosphate epimerase
-
 Escherichia coli
dihydroneopterin triphosphate epimerase
-
 Pseudomonas aeruginosa

General Information

General Information Comment Organism
malfunction deletion of gene folX selectively eliminates tetrahydromonapterin production Escherichia coli
metabolism tetrahydromonapterin formation requires both FolX and FolM, a dihydrofolate and dihydrobiopterin reductase. Tetrahydromonapterin is the physiological cofactor for phenylalanine hydroxylase, and tetrahydromonapterin can outrank folate as an end product of pterin biosynthesis, pterin pathway overview Escherichia coli
metabolism tetrahydromonapterin formation requires both FolX and FolM, a dihydrofolate and dihydrobiopterin reductase. Tetrahydromonapterin is the physiological cofactor for phenylalanine hydroxylase, and tetrahydromonapterin can outrank folate as an end product of pterin biosynthesis, pterin pathway overview Pseudomonas aeruginosa