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Literature summary for 5.1.99.6 extracted from

  • Marbaix, A.; Noël, G.; Detroux, A.; Vertommen, D.; Van Schaftingen, E.; Linster, C.
    Extremely conserved ATP- or ADP-dependent enzymatic system for nicotinamide nucleotide (2011), J. Biol. Chem., 286, 41246-41252.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.1.99.6

Activating Compound

Activating Compound Comment Organism Structure
additional information NaCl does not affect activity up to a concentration of 5 mM Mus musculus
additional information NaCl does not affect activity up to a concentration of 5 mM Saccharomyces cerevisiae
NH4Cl stimulatory effect on enzyme activity Mus musculus
NH4Cl stimulatory effect on enzyme activity Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mus musculus
expressed in Escherichia coli BL21(DE3) cells Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00033
-
 (R)-NADPH-hydrate in the presence of 5 mM KCl, at 30°C in 25 mM Tris-HCl, pH 8.0, 2 mM MgCl2, 0.2 mM ATP Mus musculus
0.0016
-
 (R)-NADH-hydrate in the presence of 5 mM KCl, at 30°C in 25 mM Tris-HCl, pH 8.0, 2 mM MgCl2, 0.2 mM ATP Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+- or Co2+-charged column chromatography and Q-Sepharose column chromatography Mus musculus
Ni2+- or Co2+-charged column chromatography and Q-Sepharose column chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-NADH-hydrate
-
 Mus musculus (S)-NADH-hydrate
-
 ?
(R)-NADH-hydrate
-
 Saccharomyces cerevisiae (S)-NADH-hydrate
-
 ?
(R)-NADPH-hydrate
-
 Saccharomyces cerevisiae (S)-NADPH-hydrate
-
 ?
(R)-NADPH-hydrate higher catalytic efficiency with (R)-NADPH-hydrate compared to(R)-NADH-hydrate Mus musculus (S)-NADPH-hydrate
-
 ?

Synonyms

Synonyms Comment Organism
AIBP
-
 Mus musculus
apolipoprotein A-1-binding protein
-
 Mus musculus
NAD(P)HX epimerase
-
 Mus musculus
NAD(P)HX epimerase
-
 Saccharomyces cerevisiae
YNL200C
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
82
-
 (R)-NADH-hydrate in the presence of 5 mM KCl, at 30°C in 25 mM Tris-HCl, pH 8.0, 2 mM MgCl2, 0.2 mM ATP Mus musculus
1827
-
 (R)-NADPH-hydrate in the presence of 5 mM KCl, at 30°C in 25 mM Tris-HCl, pH 8.0, 2 mM MgCl2, 0.2 mM ATP Mus musculus