Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.1.99.5 extracted from

  • Martinez-Rodriguez, S.; Las Heras-Vazquez, F.J.; Clemente-Jimenez, J.M.; Rodriguez-Vico, F.
    Biochemical characterization of a novel hydantoin racemase from Agrobacterium tumefaciens C58 (2004), Biochimie, 86, 77-81.
    View publication on PubMed
Search for more literature for 5.1.99.5

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Agrobacterium tumefaciens

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ strong inhibition Agrobacterium tumefaciens
Hg2+ strong inhibition Agrobacterium tumefaciens
additional information not inhibitory: EDTA, dithiothreitol Agrobacterium tumefaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.02
-
 L-isobutylhydantoin pH 7.5, 40°C Agrobacterium tumefaciens
6.3
-
 D-5-methylthioethylhydantoin pH 7.5, 40°C Agrobacterium tumefaciens
6.79
-
 D-isobutylhydantoin pH 7.5, 40°C Agrobacterium tumefaciens
10.9
-
 L-5-methylthioethylhydantoin pH 7.5, 40°C Agrobacterium tumefaciens
12.54
-
 D-ethylhydantoin pH 7.5, 40°C Agrobacterium tumefaciens
18.4
-
 L-benzylhydantoin pH 7.5, 40°C Agrobacterium tumefaciens
19.42
-
 L-ethylhydantoin pH 7.5, 40°C Agrobacterium tumefaciens
20.8
-
 D-benzylhydantoin pH 7.5, 40°C Agrobacterium tumefaciens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 4 * 27000, SDS-PAGE Agrobacterium tumefaciens
100000
-
 gel filtration Agrobacterium tumefaciens

Organism

Organism UniProt Comment Textmining
Agrobacterium tumefaciens
-
-
-
Agrobacterium tumefaciens C58 / ATCC 33970
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Agrobacterium tumefaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-5-methylthioethylhydantoin
-
 Agrobacterium tumefaciens D-5-methylthioethylhydantoin
-
 r
L-5-methylthioethylhydantoin
-
 Agrobacterium tumefaciens C58 / ATCC 33970 D-5-methylthioethylhydantoin
-
 r
L-benzylhydantoin slow racemization Agrobacterium tumefaciens D-benzylhydantoin
-
 r
L-benzylhydantoin slow racemization Agrobacterium tumefaciens C58 / ATCC 33970 D-benzylhydantoin
-
 r
L-ethylhydantoin
-
 Agrobacterium tumefaciens D-ethylhydantoin
-
 r
L-ethylhydantoin
-
 Agrobacterium tumefaciens C58 / ATCC 33970 D-ethylhydantoin
-
 r
L-isobutylhydantoin
-
 Agrobacterium tumefaciens D-isobutylhydantoin
-
 r
L-isobutylhydantoin
-
 Agrobacterium tumefaciens C58 / ATCC 33970 D-isobutylhydantoin
-
 r
additional information preference for hydantoins with short rather than long aliphatic side chains or hydantoins with aromatic rings Agrobacterium tumefaciens ?
-
 ?
additional information preference for hydantoins with short rather than long aliphatic side chains or hydantoins with aromatic rings Agrobacterium tumefaciens C58 / ATCC 33970 ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 27000, SDS-PAGE Agrobacterium tumefaciens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Agrobacterium tumefaciens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.18
-
 L-benzylhydantoin pH 7.5, 40°C Agrobacterium tumefaciens
0.46
-
 D-benzylhydantoin pH 7.5, 40°C Agrobacterium tumefaciens
0.48
-
 L-isobutylhydantoin pH 7.5, 40°C Agrobacterium tumefaciens
0.5
-
 D-5-methylthioethylhydantoin pH 7.5, 40°C Agrobacterium tumefaciens
0.78
-
 L-5-methylthioethylhydantoin pH 7.5, 40°C Agrobacterium tumefaciens
0.83
-
 D-isobutylhydantoin pH 7.5, 40°C Agrobacterium tumefaciens
1.8
-
 D-ethylhydantoin pH 7.5, 40°C Agrobacterium tumefaciens
1.81
-
 L-ethylhydantoin pH 7.5, 40°C Agrobacterium tumefaciens

pH Range

pH Minimum pH Maximum Comment Organism
7.5
-
-
 Agrobacterium tumefaciens