Literature summary for 5.1.99.4 extracted from

Savolainen, K.; Bhaumik, P.; Schmitz, W.; Kotti, T.J.; Conzelmann, E.; Wierenga, R.K.; Hiltunen, J.K.
alpha-Methylacyl-CoA racemase from Mycobacterium tuberculosis. Mutational and structural characterization of the active site and the fold (2005), J. Biol. Chem., 280, 12611-12620.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structure at 1.8 A resolution	Mycobacterium tuberculosis

Protein Variants

Protein Variants	Comment	Organism
C297A	mutant with 6.2% relative enzyme activity	Mycobacterium tuberculosis
D127A	mutant with 21.6% relative enzyme activity	Mycobacterium tuberculosis
D156A	mutant with 17.6% relative enzyme activity	Mycobacterium tuberculosis
D185A	mutant with 68.5% relative enzyme activity	Mycobacterium tuberculosis
D190A	mutant with 3.3% relative enzyme activity	Mycobacterium tuberculosis
D231A	mutant with 25.0% relative enzyme activity	Mycobacterium tuberculosis
D28A	mutant with 24.2% relative enzyme activity	Mycobacterium tuberculosis
D60A	mutant with 22.7% relative enzyme activity	Mycobacterium tuberculosis
D78A	mutant with 44.0% relative enzyme activity	Mycobacterium tuberculosis
E241A	mutant with 2.1% relative enzyme activity	Mycobacterium tuberculosis
E306A	mutant with 23.1% relative enzyme activity	Mycobacterium tuberculosis
E82A	mutant with 12.5% relative enzyme activity	Mycobacterium tuberculosis
E90A	mutant with 67.0% relative enzyme activity	Mycobacterium tuberculosis
E97A	mutant with 95.4% relative enzyme activity	Mycobacterium tuberculosis
H126A	mutant with 4.5% relative enzyme activity	Mycobacterium tuberculosis
H312A	mutant with 10.1% relative enzyme activity	Mycobacterium tuberculosis
I56P	mutant with 28.8% relative enzyme activity	Mycobacterium tuberculosis
K62A	mutant with 117.6% relative enzyme activity	Mycobacterium tuberculosis
M111P	mutant with 5.2% relative enzyme activity	Mycobacterium tuberculosis
R175A	mutant with 63.6% relative enzyme activity	Mycobacterium tuberculosis
R38A	mutant with 20.9% relative enzyme activity	Mycobacterium tuberculosis
R52A	mutant with 15.7% relative enzyme activity	Mycobacterium tuberculosis
R54A	mutant with 37.4% relative enzyme activity	Mycobacterium tuberculosis
R85A	mutant with 20.3% relative enzyme activity	Mycobacterium tuberculosis
R91A	mutant with 19.9% relative enzyme activity, 9fold increase of Km compared with the wild type	Mycobacterium tuberculosis
Y130A	mutant with 24.7% relative enzyme activity	Mycobacterium tuberculosis
Y224A	mutant with 22.6% relative enzyme activity	Mycobacterium tuberculosis
Y227A	mutant with 20.1% relative enzyme activity	Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	similar Km values for mutants H126A, D156A and E241A as for the wild-type	Mycobacterium tuberculosis
0.041	-	(R)-pristanoyl-CoA	wild type	Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
39000	-	2 * 39000, SDS-PAGE	Mycobacterium tuberculosis
89000	-	gel filtration	Mycobacterium tuberculosis

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	Vmax 214 units/mg wild-type, less than 0.1 unit/mg mutants H126A, D156A and E241A	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(R)-pristanoyl-CoA	-	Mycobacterium tuberculosis	(S)-pristanoyl-CoA	-	?
(S)-2-Methylmyristoyl-CoA	-	Mycobacterium tuberculosis	(R)-2-Methylmyristoyl-CoA	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 39000, SDS-PAGE	Mycobacterium tuberculosis

Synonyms

Synonyms	Comment	Organism
AMACR	-	Mycobacterium tuberculosis