Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.1.99.3 extracted from

  • French, J.B.; Neau, D.B.; Ealick, S.E.
    Characterization of the structure and function of Klebsiella pneumoniae allantoin racemase (2011), J. Mol. Biol., 410, 447-460.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.1.99.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli B834(DE3) cells Klebsiella pneumoniae subsp. pneumoniae

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 24-34% (w/v) polyethylene glycol 400 with 0.1 M sodium acetate, pH 3.7-4.1 Klebsiella pneumoniae subsp. pneumoniae

Protein Variants

Protein Variants Comment Organism
C184S the mutation leads to total loss of activity Klebsiella pneumoniae subsp. pneumoniae
C79S the mutation leads to total loss of activity Klebsiella pneumoniae subsp. pneumoniae
C79S/C184S the double mutation leads to total loss of activity Klebsiella pneumoniae subsp. pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 (S)-allantoin in 10 mM NaCl and 10 mM HEPES, pH 7.5, at 25°C Klebsiella pneumoniae subsp. pneumoniae
0.4
-
 (R)-allantoin in 10 mM NaCl and 10 mM HEPES, pH 7.5, at 25°C Klebsiella pneumoniae subsp. pneumoniae

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae subsp. pneumoniae
-
-
-
Klebsiella pneumoniae subsp. pneumoniae MGH78578
-
-
-
Klebsiella pneumoniae subsp. pneumoniae Trevisan MGH78578
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography, gel filtration Klebsiella pneumoniae subsp. pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-allantoin
-
 Klebsiella pneumoniae subsp. pneumoniae (S)-allantoin
-
 r
(R)-allantoin
-
 Klebsiella pneumoniae subsp. pneumoniae Trevisan MGH78578 (S)-allantoin
-
 r
(R)-allantoin
-
 Klebsiella pneumoniae subsp. pneumoniae MGH78578 (S)-allantoin
-
 r
(S)-allantoin
-
 Klebsiella pneumoniae subsp. pneumoniae (R)-allantoin
-
 r
(S)-allantoin
-
 Klebsiella pneumoniae subsp. pneumoniae Trevisan MGH78578 (R)-allantoin
-
 r
(S)-allantoin
-
 Klebsiella pneumoniae subsp. pneumoniae MGH78578 (R)-allantoin
-
 r

Subunits

Subunits Comment Organism
hexamer trimer of dimers, X-ray crystallography Klebsiella pneumoniae subsp. pneumoniae

Synonyms

Synonyms Comment Organism
HpxA
-
 Klebsiella pneumoniae subsp. pneumoniae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
183
-
 (S)-allantoin in 10 mM NaCl and 10 mM HEPES, pH 7.5, at 25°C Klebsiella pneumoniae subsp. pneumoniae
238
-
 (R)-allantoin in 10 mM NaCl and 10 mM HEPES, pH 7.5, at 25°C Klebsiella pneumoniae subsp. pneumoniae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
595
-
 (R)-allantoin in 10 mM NaCl and 10 mM HEPES, pH 7.5, at 25°C Klebsiella pneumoniae subsp. pneumoniae
610
-
 (S)-allantoin in 10 mM NaCl and 10 mM HEPES, pH 7.5, at 25°C Klebsiella pneumoniae subsp. pneumoniae