Cloned (Comment) | Organism |
---|---|
expression in Eschserichia coli | Cereibacter sphaeroides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 2 mM, complete loss of activity | Cereibacter sphaeroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
(2S)-ethylmalonyl-CoA | - |
Cereibacter sphaeroides | |
0.08 | - |
(S)-methylmalonyl-CoA | - |
Cereibacter sphaeroides |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 0.4 mM, 4fold increase in activity. Presence of Co2+ restores activity after incubation with EDTA | Cereibacter sphaeroides | |
Mn2+ | presence of Mn2+ restores activity after incubation with EDTA | Cereibacter sphaeroides | |
additional information | no activation of enzyme with Mg2+, Ni2+ | Cereibacter sphaeroides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Cereibacter sphaeroides | enzyme catalyzes the transformation of ethylmalonyl-CoA to methylsuccinyl-CoA in combination with ethylmalonyl-CoA mutase, in a ethylmalonyl-CoA dependent acyl-CoA pathway for acetate assimilation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cereibacter sphaeroides | Q3IZP4 | promiscuous ethylmalonyl-CoA/methylmalonyl-CoA epimerase | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Cereibacter sphaeroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2S)-ethylmalonyl-CoA | - |
Cereibacter sphaeroides | (2R)-ethylmalonyl-CoA | - |
? | |
(S)-methylmalonyl-CoA | - |
Cereibacter sphaeroides | (R)-methylmalonyl-CoA | - |
? | |
additional information | enzyme catalyzes the transformation of ethylmalonyl-CoA to methylsuccinyl-CoA in combination with ethylmalonyl-CoA mutase, in a ethylmalonyl-CoA dependent acyl-CoA pathway for acetate assimilation | Cereibacter sphaeroides | ? | - |
? |