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Literature summary for 5.1.99.1 extracted from

  • Erb, T.J.; Retey, J.; Fuchs, G.; Alber, B.E.
    Ethylmalonyl-CoA mutase from Rhodobacter sphaeroides defines a new subclade of coenzyme B12-dependent acyl-CoA mutases (2008), J. Biol. Chem., 283, 32283-32293.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Eschserichia coli Cereibacter sphaeroides

Inhibitors

Inhibitors Comment Organism Structure
EDTA 2 mM, complete loss of activity Cereibacter sphaeroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
(2S)-ethylmalonyl-CoA
-
Cereibacter sphaeroides
0.08
-
(S)-methylmalonyl-CoA
-
Cereibacter sphaeroides

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 0.4 mM, 4fold increase in activity. Presence of Co2+ restores activity after incubation with EDTA Cereibacter sphaeroides
Mn2+ presence of Mn2+ restores activity after incubation with EDTA Cereibacter sphaeroides
additional information no activation of enzyme with Mg2+, Ni2+ Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Cereibacter sphaeroides enzyme catalyzes the transformation of ethylmalonyl-CoA to methylsuccinyl-CoA in combination with ethylmalonyl-CoA mutase, in a ethylmalonyl-CoA dependent acyl-CoA pathway for acetate assimilation ?
-
?

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides Q3IZP4 promiscuous ethylmalonyl-CoA/methylmalonyl-CoA epimerase
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S)-ethylmalonyl-CoA
-
Cereibacter sphaeroides (2R)-ethylmalonyl-CoA
-
?
(S)-methylmalonyl-CoA
-
Cereibacter sphaeroides (R)-methylmalonyl-CoA
-
?
additional information enzyme catalyzes the transformation of ethylmalonyl-CoA to methylsuccinyl-CoA in combination with ethylmalonyl-CoA mutase, in a ethylmalonyl-CoA dependent acyl-CoA pathway for acetate assimilation Cereibacter sphaeroides ?
-
?