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Literature summary for 5.1.99.1 extracted from
- DL-Methylmalonyl-CoA racemase from rat liver (1988), Methods Enzymol., 166, 400-406.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | - |
Rattus norvegicus |  |
| Cu2+ | - |
Rattus norvegicus | |
| EDTA | reactivation by Co2+ | Rattus norvegicus | |
| Zn2+ | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
DL-methylmalonyl-CoA | - |
Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | increases activity | Rattus norvegicus | |
| Co3+ | lower level of activation than Co2+ at higher concentrations | Rattus norvegicus | |
| Fe2+ | lower level of activation than Co2+ at higher concentrations | Rattus norvegicus | |
| Mn2+ | lower level of activation than Co2+ at higher concentrations | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 16000 | - |
2 * 16000, subunits not connected by disulfide bonds, SDS-PAGE in presence and absence of 2-mercaptoethanol | Rattus norvegicus |
| 32000 | - |
gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8400 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-2-Methyl-3-oxopropanoyl-CoA | - |
Rattus norvegicus | (S)-2-Methyl-3-oxopropanoyl-CoA | - |
? | |
| DL-methylmalonyl-CoA | - |
Rattus norvegicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 16000, subunits not connected by disulfide bonds, SDS-PAGE in presence and absence of 2-mercaptoethanol | Rattus norvegicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4170 | - |
DL-methylmalonyl-CoA | - |
Rattus norvegicus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | 50% of maximal activity at pH 5.5 and 8.5 | Rattus norvegicus |
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Release 2023.1 (January 2023)
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