Protein Variants | Comment | Organism |
---|---|---|
E156D | mutant exhibits 63% of the wild-type activity | Agrobacterium tumefaciens |
R215K | mutant exhibits 25% of the wild-type activity | Agrobacterium tumefaciens |
W112A | mutant enzyme exhibits no detectable activity | Agrobacterium tumefaciens |
W112F | mutant enzyme displays 19% of the wild type enzyme activity. kcat/Km for D-fructose is 100fold lower than wild-type value, kcat/Km fur D-psicose is 84fold lower than wild-type value | Agrobacterium tumefaciens |
W112H | mutant enzyme displays 65% of the wild type enzyme activity | Agrobacterium tumefaciens |
W112Y | mutant enzyme displays 54% of the wild type enzyme activity | Agrobacterium tumefaciens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | 3 | D-psicose | pH 8.0, 45°C, wild-type enzyme | Agrobacterium tumefaciens | |
33 | - |
D-fructose | pH 8.0, 45°C, wild-type enzyme | Agrobacterium tumefaciens | |
37 | - |
D-psicose | pH 8.0, 45°C, mutant enzyme R215K | Agrobacterium tumefaciens | |
48 | - |
D-psicose | pH 8.0, 45°C, mutant enzyme E156D | Agrobacterium tumefaciens | |
100 | - |
D-fructose | pH 8.0, 45°C, mutant enzyme D183E | Agrobacterium tumefaciens | |
100 | - |
D-psicose | pH 8.0, 45°C, mutant enzyme W112F | Agrobacterium tumefaciens | |
139 | - |
D-fructose | pH 8.0, 45°C, mutant enzyme E156D | Agrobacterium tumefaciens | |
270 | - |
D-fructose | pH 8.0, 45°C, mutant enzyme W112F | Agrobacterium tumefaciens | |
309 | - |
D-fructose | pH 8.0, 45°C, mutant enzyme R215K | Agrobacterium tumefaciens | |
427 | - |
D-fructose | pH 8.0, 45°C, mutant enzyme H209A | Agrobacterium tumefaciens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | the distances between the catalytic residues (Glu150 and Glu244) and Mn2+ are critical to the catalysis, and the negative charges of the metal-binding residues are important for interaction with metal ion | Agrobacterium tumefaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrobacterium tumefaciens | A9CH28 | - |
- |
Agrobacterium tumefaciens ATCC 33970 | A9CH28 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose | - |
Agrobacterium tumefaciens | D-psicose | - |
r | |
D-fructose | - |
Agrobacterium tumefaciens ATCC 33970 | D-psicose | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
assay at | Agrobacterium tumefaciens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
D-fructose | pH 8.0, 45°C, mutant enzyme D183E | Agrobacterium tumefaciens | |
4.9 | - |
D-fructose | pH 8.0, 45°C, mutant enzyme R215K | Agrobacterium tumefaciens | |
7.7 | - |
D-fructose | pH 8.0, 45°C, mutant enzyme H209A | Agrobacterium tumefaciens | |
17 | - |
D-fructose | pH 8.0, 45°C, mutant enzyme E156D | Agrobacterium tumefaciens | |
23 | - |
D-psicose | pH 8.0, 45°C, mutant enzyme R215K | Agrobacterium tumefaciens | |
26 | - |
D-fructose | pH 8.0, 45°C, mutant enzyme W112F | Agrobacterium tumefaciens | |
44 | - |
D-psicose | pH 8.0, 45°C, mutant enzyme W112F | Agrobacterium tumefaciens | |
46 | - |
D-psicose | pH 8.0, 45°C, mutant enzyme E156D | Agrobacterium tumefaciens | |
341 | - |
D-fructose | pH 8.0, 45°C, wild-type enzyme | Agrobacterium tumefaciens | |
478 | - |
D-psicose | pH 8.0, 45°C, wild-type enzyme | Agrobacterium tumefaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Agrobacterium tumefaciens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00029 | - |
D-fructose | pH 8.0, 45°C, mutant enzyme D183E | Agrobacterium tumefaciens | |
0.015 | - |
D-fructose | pH 8.0, 45°C, mutant enzyme R215K | Agrobacterium tumefaciens | |
0.018 | - |
D-fructose | pH 8.0, 45°C, mutant enzyme H209A | Agrobacterium tumefaciens | |
0.1 | - |
D-fructose | pH 8.0, 45°C, mutant enzyme W112F | Agrobacterium tumefaciens | |
0.12 | - |
D-fructose | pH 8.0, 45°C, mutant enzyme E156D | Agrobacterium tumefaciens | |
0.44 | - |
D-psicose | pH 8.0, 45°C, mutant enzyme W112F | Agrobacterium tumefaciens | |
0.62 | - |
D-psicose | pH 8.0, 45°C, mutant enzyme R215K | Agrobacterium tumefaciens | |
0.96 | - |
D-psicose | pH 8.0, 45°C, mutant enzyme E156D | Agrobacterium tumefaciens | |
10 | - |
D-fructose | pH 8.0, 45°C, wild-type enzyme | Agrobacterium tumefaciens | |
37 | - |
D-psicose | pH 8.0, 45°C, wild-type enzyme | Agrobacterium tumefaciens |