Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Anabaena sp. CH1 |
Crystallization (Comment) | Organism |
---|---|
of its apoform | Anabaena sp. CH1 |
Protein Variants | Comment | Organism |
---|---|---|
C370A | site directed mutagenesis | Anabaena sp. CH1 |
E218A | site directed mutagenesis | Anabaena sp. CH1 |
E218D | site directed mutagenesis | Anabaena sp. CH1 |
E242Q | site directed mutagenesis | Anabaena sp. CH1 |
E308A | site directed mutagenesis | Anabaena sp. CH1 |
E308D | site directed mutagenesis | Anabaena sp. CH1 |
H239A | site directed mutagenesis | Anabaena sp. CH1 |
H239N | site directed mutagenesis | Anabaena sp. CH1 |
H372A | site directed mutagenesis | Anabaena sp. CH1 |
H372I | site directed mutagenesis | Anabaena sp. CH1 |
H372N | site directed mutagenesis | Anabaena sp. CH1 |
N179A | site directed mutagenesis | Anabaena sp. CH1 |
R375A | site directed mutagenesis | Anabaena sp. CH1 |
R375K | site directed mutagenesis | Anabaena sp. CH1 |
R57A | site directed mutagenesis | Anabaena sp. CH1 |
R57K | site directed mutagenesis | Anabaena sp. CH1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.2 | - |
N-acetyl-D-glucosamine | E242Q, reverse conversion in the presence of 1 mM ATP | Anabaena sp. CH1 | |
7.4 | - |
N-acetyl-D-glucosamine | R375K, reverse conversion in the presence of 1 mM ATP | Anabaena sp. CH1 | |
9.2 | - |
N-acetyl-D-mannosamine | R375K, in the presence of 1 mM ATP | Anabaena sp. CH1 | |
13.4 | - |
N-acetyl-D-glucosamine | E218D, reverse conversion in the presence of 1 mM ATP | Anabaena sp. CH1 | |
13.9 | - |
N-acetyl-D-mannosamine | R375A, in the presence of 1 mM ATP | Anabaena sp. CH1 | |
14.8 | - |
N-acetyl-D-mannosamine | wild type, at pH 7, in the presence of 1 mM ATP | Anabaena sp. CH1 | |
15.5 | - |
N-acetyl-D-mannosamine | E218A, in the presence of 1 mM ATP | Anabaena sp. CH1 | |
15.6 | - |
N-acetyl-D-mannosamine | wild type, at pH 8, in the presence of 1 mM ATP | Anabaena sp. CH1 | |
16.7 | - |
N-acetyl-D-glucosamine | C370A, reverse conversion in the presence of 1 mM ATP | Anabaena sp. CH1 | |
16.7 | - |
N-acetyl-D-glucosamine | R375A, reverse conversion in the presence of 1 mM ATP | Anabaena sp. CH1 | |
21 | - |
N-acetyl-D-mannosamine | E218D, in the presence of 1 mM ATP | Anabaena sp. CH1 | |
21.3 | - |
N-acetyl-D-mannosamine | C370A, in the presence of 1 mM ATP | Anabaena sp. CH1 | |
24.4 | - |
N-acetyl-D-glucosamine | E218A, reverse conversion in the presence of 1 mM ATP | Anabaena sp. CH1 | |
25.4 | - |
N-acetyl-D-glucosamine | wild type, reverse conversion in the presence of 1 mM ATP | Anabaena sp. CH1 | |
27.2 | - |
N-acetyl-D-mannosamine | N179A, in the presence of 1 mM ATP | Anabaena sp. CH1 | |
32.7 | - |
N-acetyl-D-mannosamine | wild type, at pH 9, in the presence of 1 mM ATP | Anabaena sp. CH1 | |
45.7 | - |
N-acetyl-D-glucosamine | N179A, reverse conversion in the presence of 1 mM ATP | Anabaena sp. CH1 | |
53.8 | - |
N-acetyl-D-mannosamine | E242Q, in the presence of 1 mM ATP | Anabaena sp. CH1 | |
57.33 | - |
N-acetyl-D-mannosamine | wild type, at pH 6 in the presence of 1 mM ATP | Anabaena sp. CH1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anabaena sp. CH1 | A4UA16 | - |
- |
Purification (Comment) | Organism |
---|---|
of the recombinant proteins, wild type and mutants by immobilized nickel-ion chromatography | Anabaena sp. CH1 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.6 | - |
E242Q, reverse conversion in the presence of 1 mM ATP | Anabaena sp. CH1 |
6.2 | - |
R375A, reverse conversion in the presence of 1 mM ATP | Anabaena sp. CH1 |
6.6 | - |
E218A, reverse conversion in the presence of 1 mM ATP | Anabaena sp. CH1 |
8.1 | - |
E242Q, in the presence of 1 mM ATP | Anabaena sp. CH1 |
8.9 | - |
R375K, reverse conversion in the presence of 1 mM ATP | Anabaena sp. CH1 |
22.8 | - |
E218A, in the presence of 1 mM ATP | Anabaena sp. CH1 |
46.5 | - |
E218D, reverse conversion in the presence of 1 mM ATP | Anabaena sp. CH1 |
50.8 | - |
N179A, reverse conversion in the presence of 1 mM ATP | Anabaena sp. CH1 |
54.8 | - |
R375A, in the presence of 1 mM ATP | Anabaena sp. CH1 |
85.9 | - |
C370A, reverse conversion in the presence of 1 mM ATP | Anabaena sp. CH1 |
94.3 | - |
R375K, in the presence of 1 mM ATP | Anabaena sp. CH1 |
123.8 | - |
wild type, reverse conversion in the presence of 1 mM ATP | Anabaena sp. CH1 |
256.4 | - |
E218D, in the presence of 1 mM ATP | Anabaena sp. CH1 |
258.5 | - |
N179A, in the presence of 1 mM ATP | Anabaena sp. CH1 |
351 | - |
C370A, in the presence of 1 mM ATP | Anabaena sp. CH1 |
525.8 | - |
wild type, in the presence of 1 mM ATP | Anabaena sp. CH1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-mannosamine | - |
Anabaena sp. CH1 | N-acetyl-D-glucosamine | - |
r |
Synonyms | Comment | Organism |
---|---|---|
bGlcNAc 2-epimerase | recombinant protein | Anabaena sp. CH1 |
GlcNAc 2-epimerase | - |
Anabaena sp. CH1 |
N-acyl-D-glucosamine 2-epimerase | - |
Anabaena sp. CH1 |
N-acylglucosamine 2-epimerase | - |
Anabaena sp. CH1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Anabaena sp. CH1 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9.5 | - |
Anabaena sp. CH1 |