Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Bacillus subtilis | |
glucosamine | competitive with UDP-N-acetylglucosamine | Bacillus subtilis | |
HgCl2 | - |
Bacillus subtilis | |
iodoacetate | - |
Bacillus subtilis | |
NEM | - |
Bacillus subtilis | |
PCMB | - |
Bacillus subtilis | |
Zn2+ | - |
Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.4 | - |
UDP-N-acetylglucosamine | - |
Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stimulates | Bacillus subtilis | |
Co2+ | stimulates | Bacillus subtilis | |
Mg2+ | stimulates | Bacillus subtilis | |
Mn2+ | stimulates | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
gel filtration | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
565 | - |
- |
Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine | - |
Bacillus subtilis | UDP-N-acetyl-D-galactosamine | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 35 | without NAD+ | Bacillus subtilis |
40 | 45 | in presence of 1 mM NAD+ | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
pH 7.5, 15 min, 65% loss of activity in presence of 1 mM NAD+, in absence of NAD+ complete loss of activity | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | - |
Bacillus subtilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | 4°C, stable | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | highly stimulated by small amounts, Km: 0.13 mM | Bacillus subtilis |