Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Azotobacter vinelandii |
Protein Variants | Comment | Organism |
---|---|---|
additional information | division of algE1 into two parts based on the modular type of structure, and expression of each part in Escherichia coli. AlgE1 contains two catalytic domains, AlgE1-1, which introduces both G-blocks and MG-blocks, and AlgE1-2, which only introduces MG-blocks. AlgE1-1 has a much lower specific activity than both AlgE1-2 and AlgE1 | Azotobacter vinelandii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required, optimum concentration 0.8 mM. The Ca2+ requirement of domain AlgE1-1 is much higher (optimum around 3 mM) than that of whole AlgE1, while domain AlgE1-2 needs slightly more Ca2+ than AlgE1 for optimal activity | Azotobacter vinelandii | |
Mg2+ | at Ca2+ concentrations lower than optimum, the addition of Sr2+ or Mg2+ has a stimulatory effect, but Mg2+ is not able to substitute for Ca2+ when no calcium is added | Azotobacter vinelandii | |
Sr2+ | may partly substitute for Ca2+. At Ca2+ concentrations lower than optimum, the addition of Sr2+ or Mg21 has a stimulatory effect | Azotobacter vinelandii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azotobacter vinelandii | Q44494 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.37 | - |
catalytic domain AlgE1-1, pH 6.9, 37°C | Azotobacter vinelandii |
4.4 | - |
catalytic domain AlgE1-2, pH 6.9, 37°C | Azotobacter vinelandii |
4.9 | - |
pH 6.9, 37°C | Azotobacter vinelandii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[alginate]-beta-D-mannuronate | - |
Azotobacter vinelandii | [alginate]-alpha-L-guluronate | reaction product of AlgE1 is a mixture of blocks of continuous G residues (G-blocks) and blocks containing alternating M and G residues (MG-blocks) | ? |