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Literature summary for 5.1.3.37 extracted from

  • Ertesvag, H.; Hoidal, H.K.; Skjak-Braek, G.; Valla, S.
    The Azotobacter vinelandii mannuronan C-5-epimerase AlgE1 consists of two separate catalytic domains (1998), J. Biol. Chem., 273, 30927-30932.
    View publication on PubMed
Search for more literature for 5.1.3.37

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Azotobacter vinelandii

Protein Variants

Protein Variants Comment Organism
additional information division of algE1 into two parts based on the modular type of structure, and expression of each part in Escherichia coli. AlgE1 contains two catalytic domains, AlgE1-1, which introduces both G-blocks and MG-blocks, and AlgE1-2, which only introduces MG-blocks. AlgE1-1 has a much lower specific activity than both AlgE1-2 and AlgE1 Azotobacter vinelandii

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required, optimum concentration 0.8 mM. The Ca2+ requirement of domain AlgE1-1 is much higher (optimum around 3 mM) than that of whole AlgE1, while domain AlgE1-2 needs slightly more Ca2+ than AlgE1 for optimal activity Azotobacter vinelandii
Mg2+ at Ca2+ concentrations lower than optimum, the addition of Sr2+ or Mg2+ has a stimulatory effect, but Mg2+ is not able to substitute for Ca2+ when no calcium is added Azotobacter vinelandii
Sr2+ may partly substitute for Ca2+. At Ca2+ concentrations lower than optimum, the addition of Sr2+ or Mg21 has a stimulatory effect Azotobacter vinelandii

Organism

Organism UniProt Comment Textmining
Azotobacter vinelandii Q44494
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.37
-
 catalytic domain AlgE1-1, pH 6.9, 37°C Azotobacter vinelandii
4.4
-
 catalytic domain AlgE1-2, pH 6.9, 37°C Azotobacter vinelandii
4.9
-
 pH 6.9, 37°C Azotobacter vinelandii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[alginate]-beta-D-mannuronate
-
 Azotobacter vinelandii [alginate]-alpha-L-guluronate reaction product of AlgE1 is a mixture of blocks of continuous G residues (G-blocks) and blocks containing alternating M and G residues (MG-blocks) ?