Application | Comment | Organism |
---|---|---|
analysis | in vitro assay for mannuronan C5 epimerization wherein extracts of Escherichia coli expressing high levels of AlgG are incubated with polymannuronate. Epimerization of D-mannuronate to L-guluronate residues in the polymer is detected enzymatically, using a L-guluronate-specific alginate lyase of Klebsieila aerogenes | Pseudomonas aeruginosa |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas aeruginosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Pseudomonas aeruginosa | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
x * 60000, preform, x * 55000, mature protein, SDS-PAGE | Pseudomonas aeruginosa |
60000 | - |
x * 60000, preform, x * 55000, mature protein, SDS-PAGE | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | Q51371 | isoform AlgG | - |
Pseudomonas aeruginosa ATCC 15692 | Q51371 | isoform AlgG | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | sequence containes a putative N-terminal signal sequence of 35 amino acids | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | epimerization reaction is detected only when acetyl groups are removed from the poly-D-mannuronate substrate, suggesting that AlgG epimerization activity in vivo may be sensitive to acetylation of the D-mannuronan residues | Pseudomonas aeruginosa | ? | - |
? | |
additional information | epimerization reaction is detected only when acetyl groups are removed from the poly-D-mannuronate substrate, suggesting that AlgG epimerization activity in vivo may be sensitive to acetylation of the D-mannuronan residues | Pseudomonas aeruginosa ATCC 15692 | ? | - |
? | |
[mannuronan]-beta-D-mannuronate | - |
Pseudomonas aeruginosa | [alginate]-alpha-L-guluronate | isoform AlgG acts as a polymer-level mannuronan C5-epimerase | ? | |
[mannuronan]-beta-D-mannuronate | - |
Pseudomonas aeruginosa ATCC 15692 | [alginate]-alpha-L-guluronate | isoform AlgG acts as a polymer-level mannuronan C5-epimerase | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, preform, x * 55000, mature protein, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
algG | - |
Pseudomonas aeruginosa |