Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | [Clostridium] scindens |
General Stability | Organism |
---|---|
Mn2+ improves the structural stability during both heat- and urea-induced unfolding | [Clostridium] scindens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | 8.3 | D-psicose | pH 7.5, 60°C | [Clostridium] scindens | |
40.1 | - |
D-fructose | pH 7.5, 60°C | [Clostridium] scindens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | the enzyme is strictly metal-dependent and requires Mn2+ as optimum cofactor for activity | [Clostridium] scindens |
Organic Solvent | Comment | Organism |
---|---|---|
urea | midtransition concentration: 2.68 M | [Clostridium] scindens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Clostridium] scindens | B0NGC5 | - |
- |
[Clostridium] scindens ATCC 35704 | B0NGC5 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
[Clostridium] scindens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose | the catalytic efficiency for D-psicose is 7.4fold higher than that for D-tagatose. The equilibrium ratio between D-psicose and D-fructose is 28:72. The catalytic efficiency for D-psicose is 258fold higher than that for D-tagatose | [Clostridium] scindens | D-psicose | - |
r | |
D-fructose | the catalytic efficiency for D-psicose is 7.4fold higher than that for D-tagatose. The equilibrium ratio between D-psicose and D-fructose is 28:72. The catalytic efficiency for D-psicose is 258fold higher than that for D-tagatose | [Clostridium] scindens ATCC 35704 | D-psicose | - |
r | |
D-psicose | the catalytic efficiency for D-psicose is 7.4fold higher than that for D-tagatose. The equilibrium ratio between D-psicose and D-fructose is 28:72. The catalytic efficiency for D-psicose is 258fold higher than that for D-tagatose | [Clostridium] scindens | D-fructose | - |
r | |
D-psicose | the catalytic efficiency for D-psicose is 7.4fold higher than that for D-tagatose. The equilibrium ratio between D-psicose and D-fructose is 28:72. The catalytic efficiency for D-psicose is 258fold higher than that for D-tagatose | [Clostridium] scindens ATCC 35704 | D-fructose | - |
r |
Synonyms | Comment | Organism |
---|---|---|
CLOSCI_02528 | locus name | [Clostridium] scindens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
[Clostridium] scindens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 75 | 35°C: about 45% of maximal activity, 75°C: about 55% of maximal activity | [Clostridium] scindens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
half-life: 108 min | [Clostridium] scindens |
55 | - |
1 h, about 40% loss of activity | [Clostridium] scindens |
60 | - |
1 h, about 55% loss of activity | [Clostridium] scindens |
64.4 | - |
apparent melting temperature | [Clostridium] scindens |
65 | - |
1 h, about 80% loss of activity | [Clostridium] scindens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
350 | - |
D-fructose | pH 7.5, 60°C | [Clostridium] scindens | |
1827 | - |
D-psicose | pH 7.5, 60°C | [Clostridium] scindens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
[Clostridium] scindens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | pH 5.5: about 66% of maximal activity, pH 9.0: about 65% of maximal activity | [Clostridium] scindens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
4°C, 2 h, about 30% loss of activity | [Clostridium] scindens |
6 | - |
4°C, 2 h, about 15% loss of activity | [Clostridium] scindens |
6.5 | - |
4°C, 2 h, about 15% loss of activity | [Clostridium] scindens |
7 | - |
4°C, 2 h, about 5% loss of activity | [Clostridium] scindens |
7.5 | - |
4°C, 2 h, less than 5% loss of activity | [Clostridium] scindens |
8 | - |
4°C, 2 h, about 10% loss of activity | [Clostridium] scindens |
8.5 | - |
4°C, 2 h, about 10% loss of activity | [Clostridium] scindens |
9 | - |
4°C, 2 h, about 20% loss of activity | [Clostridium] scindens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.72 | - |
D-fructose | pH 7.5, 60°C | [Clostridium] scindens | |
64.5 | - |
D-psicose | pH 7.5, 60°C | [Clostridium] scindens |